Analytical Data
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Gene name
MAP3K11
- Application
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Alternative Names
M3K11_HUMAN; Map3k11; MEKK11; MGC17114; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Mixed lineage protein kinase 3; Mlk-3; Mlk3; Protein tyrosine kinase PTK1 ; PTK1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16584
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Expression Region
1-378aa
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AA Sequence
MPRFRTRALAASGGGDHGHPEKVLHTSQRRLPAMEPLKSLFLKSPLGSWNGSGSGGGGGGGGGRPEGSPKAAGYANPVWTALFDYEPSGQDELALRKGDRVEVLSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRRVPPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIESDDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIKASTFSKGSDVWRCGPWWGWTGCYGAAEGRGGCLNLGLGQVQGQGPKPLGS
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Molecular Weight
66.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MAP3K11, also known as MEKK4 (Mitogen-Activated Protein Kinase Kinase Kinase 4), is a member of the MAP3K family, which plays crucial roles in cell signaling pathways related to stress responses, cell proliferation, and apoptosis. The protein has garnered attention due to its involvement in various physiological and pathological processes, including inflammation, cancer progression, and neurodegeneration. Research into MAP3K11 is significant as it serves as an integrative point in MAPK signaling cascades, influencing downstream effector pathways such as JNK and p38 MAPK. Given its central role, MAP3K11 is implicated in the modulation of cellular responses to environmental stresses and growth factors. The recombinant expression of MAP3K11 allows for detailed studies on its functional mechanisms, interactions with other signaling proteins, and its effects on cellular behavior. Utilizing recombinant MAP3K11 in assay systems can aid in discovering its potential as a therapeutic target, especially in diseases where MAPK signaling is aberrant. Investigations into its structure-function relationships also pave the way for the development of specific inhibitors or modulators that can influence its activity in pathological conditions. Therefore, the study of MAP3K11 not only enhances our understanding of MAPK signaling networks but also holds promise for identifying novel therapeutic strategies for diseases where this pathway is disrupted.
