Cat: PA2000-724DB

Recombinant Human DKC Protein,His

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Analytical Data

  • Gene name

    DKC

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    DKC;NOLA4;H/ACA ribonucleoProtein complex subunit DKC1

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    O60832

  • Expression Region

    2-514aa

  • AA Sequence

    ADAEVIILP KKHKKKKERK SLPEEDVAEI QHAEEFLIKP ESKVAKLDTS QWPLLLKNFD KLNVRTTHYT PLACGSNPLK REIGDYIRTG FINLDKPSNP SSHEVVAWIR RILRVEKTGH SGTLDPKVTG CLIVCIERAT RLVKSQQSAG KEYVGIVRLH NAIEGGTQLS RALETLTGAL FQRPPLIAAV KRQLRVRTIY ESKMIEYDPE RRLGIFWVSC EAGTYIRTLC VHLGLLLGVG GQMQELRRVR SGVMSEKDHM VTMHDVLDAQ WLYDNHKDES YLRRVVYPLE KLLTSHKRLV MKDSAVNAIC YGAKIMLPGV LRYEDGIEVN QEIVVITTKG EAICMAIALM TTAVISTCDH GIVAKIKRVI MERDTYPRKW GLGPKASQKK LMIKQGLLDK HGKPTDSTPA TWKQEYVDYS ESAKKEVVAE VVKAPQVVAE AAKTAKRKRE SESESDETPP AAPQLIKKEK KKSKKDKKAK AGLESGAEPG DGDSDTTKKK KKKKKAKEVE LVSE

  • Molecular Weight

    57.6 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

Related Products

Protein Description

The study of DKC (Dyskeratosis Congenita) related recombinant proteins has gained significant attention due to their association with the rare genetic disorder Dyskeratosis Congenita, characterized by symptoms such as abnormal skin pigmentation, nail dystrophy, and an increased risk of malignancies. The disorder is primarily linked to mutations in genes involved in telomere maintenance, leading to cellular aging and dysfunction. Research has focused on the role of telomerase, particularly the TERC and TERT components, and other DKC-related proteins, in mediating telomere elongation and stabilization. Recombinant proteins derived from these genes provide a valuable tool for understanding the pathogenic mechanisms underlying DKC. They are utilized in various experimental models to elucidate the molecular pathways involved in telomere biology. Additionally, these proteins hold promise for potential therapeutic interventions aimed at ameliorating the symptoms of DKC and other telomere-related diseases. By exploring the structure and function of DKC recombinant proteins, researchers aim to uncover new insights into their role in cellular aging, cancer susceptibility, and therapeutic targets for improving patient outcomes.

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