Analytical Data
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Gene name
DOK1
- Application
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Alternative Names
DOK1；Docking Protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99704
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Expression Region
1-481aa
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AA Sequence
MDGAVMEGPLFLQSQRFGTKRWRKTWAVLYPASPHGVARLEFFDHKGSSSGGGRGSSRRLDCKVIRLAECVSVAPVTVETPPEPGATAFRLDTAQRSHLLAADAPSSAAWVQTLCRNAFPKGSWTLAPTDNPPKLSALEMLENSLYSPTWEGSQFWVTVQRTEAAERCGLHGSYVLRVEAERLTLLTVGAQSQILEPLLSWPYTLLRRYGRDKVMFSFEAGRRCPSGPGTFTFQTAQGNDIFQAVETAIHRQKAQGKAGQGHDVLRADSHEGEVAEGKLPSPPGPQELLDSPPALYAEPLDSLRIAPCPSQDSLYSDPLDSTSAQAGEGVQRKKPLYWDLYEHAQQQLLKAKLTDPKEDPIYDEPEGLAPVPPQGLYDLPREPKDAWWCQARVKEEGYELPYNPATDDYAVPPPRSTKPLLAPKPQGPAFPEPGTATGSGIKSHNSALYSQVQKSGASGSWDCGLSRVGTDKTGVKSEGST
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Molecular Weight
79 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DOK1, or Docking Protein 1, plays a pivotal role in various cellular processes, including signal transduction and cellular growth regulation. As a member of the DOK family, DOK1 is characterized by the presence of specific protein-interaction domains that facilitate its role as an adapter protein in signaling pathways, primarily those involving receptor tyrosine kinases. Research into DOK1 has gained momentum due to its involvement in several physiological and pathological conditions, such as cancer, where its expression levels and functional status can influence tumor progression and response to therapy. Understanding the structure and function of DOK1 at the molecular level can elucidate its mechanisms of action and regulatory roles in cellular contexts. Furthermore, DOK1's interactions with various signaling partners make it a potential target for therapeutic interventions, which has driven efforts to characterize recombinant DOK1 protein for use in detailed biochemical and biophysical studies. By producing and studying recombinant DOK1, researchers aim to uncover its functional implications in health and disease, paving the way for novel strategies in drug development and biomarker discovery. This research is vital for the advancement of targeted therapies that could manipulate DOK1's role in disease pathways, thereby improving therapeutic outcomes in conditions where DOK1 is implicated.
