Analytical Data
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Gene name
ADD1
- Application
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Alternative Names
ADD1；ADDA；Alpha-adducin
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P35611
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Expression Region
1-737aa
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AA Sequence
MNGDSRAAVV TSPPPTTAPH KERYFDRVDE NNPEYLRERN MAPDLRQDFN MMEQKKRVSM ILQSPAFCEE LESMIQEQFK KGKNPTGLLA LQQIADFMTT NVPNVYPAAP QGGMAALNMS LGMVTPVNDL RGSDSIAYDK GEKLLRCKLA AFYRLADLFG WSQLIYNHIT TRVNSEQEHF LIVPFGLLYS EVTASSLVKI NLQGDIVDRG STNLGVNQAG FTLHSAIYAA RPDVKCVVHI HTPAGAAVSA MKCGLLPISP EALSLGEVAY HDYHGILVDE EEKVLIQKNL GPKSKVLILR NHGLVSVGES VEEAFYYIHN LVVACEIQVR TLASAGGPDN LVLLNPEKYK AKSRSPGSPV GEGTGSPPKW QIGEQEFEAL MRMLDNLGYR TGYPYRYPAL REKSKKYSDV EVPASVTGYS FASDGDSGTC SPLRHSFQKQ QREKTRWLNS GRGDEASEEG QNGSSPKSKT KWTKEDGHRT STSAVPNLFV PLNTNPKEVQ EMRNKIREQN LQDIKTAGPQ SQVLCGVVMD RSLVQGELVT ASKAIIEKEY QPHVIVSTTG PNPFTTLTDR ELEEYRREVE RKQKGSEENL DEAREQKEKS PPDQPAVPHP PPSTPIKLEE DLVPEPTTGD DSDAATFKPT LPDLSPDEPS EALGFPMLEK EEEAHRPPSP TEAPTEASPE PAPDPAPVAE EAAPSAVEEG AAADPGSDGS PGKSPSKKKK KFRTPSFLKK SKKKSDS
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Molecular Weight
80.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ADD1, or Adipocyte Differentiation-related Protein 1, is a critical transcription factor involved in the regulation of adipogenesis and metabolism. Its expression is primarily observed in adipose tissues, where it plays a significant role in the differentiation of preadipocytes into mature adipocytes, influencing lipid storage and energy homeostasis. Research on ADD1 has gained substantial attention due to its potential implications in obesity and metabolic disorders. The protein is believed to modulate insulin sensitivity and regulate various genes associated with lipid metabolism, making it a focal point in understanding the biological mechanisms underlying adipose tissue function. Studies have shown that altered expression of ADD1 can contribute to dysregulation of fat deposition and insulin resistance, which are common in conditions such as type 2 diabetes and cardiovascular diseases. Furthermore, the investigation of ADD1’s signaling pathways may provide valuable insights into therapeutic targets for obesity and related metabolic syndromes. Recent advancements in molecular biology techniques have facilitated the exploration of ADD1's role in different physiological contexts, revealing its interaction with various other transcription factors and signaling molecules. Overall, the ongoing research into ADD1 not only enhances our comprehension of adipocyte biology but also aims to uncover novel strategies for combating obesity-related health issues. Understanding its function could lead to potential interventions that modulate its activity, ultimately aiding in the development of effective treatments for obesity and associated metabolic disorders.
