Analytical Data
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基因名
MAN1A2
- Application
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别名
Mannosidase alpha class 1A member 2;Processing alpha-1.2-mannosidase IB
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O60476
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表达区间
1-641aa
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氨基酸序列
MTTPALLPLSGRRIPPLNLGPPSFPHHRATLRLSEKFILLLILSAFITLCFGAFFFLPDSSKHKRFDLGLEDVLIPHVDAGKGAKNPGVFLIHGPDEHRHREEEERLRNKIRADHEKALEEAKEKLRKSREEIRAEIQTEKNKVVQEMKIKENKPLPPVPIPNLVGIRGGDPEDNDIREKREKIKEMMKHAWDNYRTYGWGHNELRPIARKGHSPNIFGSSQMGATIVDALDTLYIMGLHDEFLDGQRWIEDNLDFSVNSEVSVFEVNIRFIGGLLAAYYLSGEEIFKIKAVQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLTFIGEWKNGHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTALKLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKYCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPLPVLHLANTTLSGNPAVR
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分子量
74.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MAN1A2 is a crucial enzyme belonging to the mannosidase family, playing a significant role in the glycosylation process, which is vital for protein folding and stability in the endoplasmic reticulum. Aberrant glycosylation has been linked to various diseases, including congenital disorders of glycosylation and certain types of cancer. Research on MAN1A2 has gained momentum due to its potential implications in these pathological conditions. The enzyme catalyzes the hydrolysis of mannose residues from glycoproteins, influencing protein maturation and turnover. Understanding the structure and function of MAN1A2 can provide insights into its mechanisms and regulatory pathways, revealing potential therapeutic targets for diseases associated with glycosylation defects. Moreover, recombinant production of MAN1A2 allows for detailed biochemical characterization and functional studies, enhancing our understanding of its activity and role in cellular processes. As the demand for biopharmaceuticals grows, elucidating MAN1A2's function could lead to advancements in therapeutic strategies, particularly in the field of glycoprotein engineering. Overall, the study of MAN1A2 is poised to contribute substantially to both basic science and applied biomedical research.
