Analytical Data
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Gene name
a4GALT
- Application
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Alternative Names
a4GALT；A14GALT；A4GALT1；Lactosylceramide 4-alpha-galactosyltransferase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NPC4
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Expression Region
1-353aa
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AA Sequence
MSKPPDLLLRLLRGAPRQRVCTLFIIGFKFTFFVSIMIYWHVVGEPKEKG QLYNLPAEIPCPTLTPPTPPSHGPTPGNIFFLETSDRTNPNFLFMCSVES AARTHPESHVLVLMKGLPGGNASLPRHLGISLLSCFPNVQMLPLDLRELF RDTPLADWYAAVQGRWEPYLLPVLSDASRIALMWKFGGIYLDTDFIVLKN LRNLTNVLGTQSRYVLNGAFLAFERRHEFMALCMRDFVDHYNGWIWGHQG PQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDIN PEELPRLLSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEAMK MYL
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Molecular Weight
67 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the A4GALT recombinant protein is centered on its role in glycosylation processes, particularly the transfer of galactose to the core structure of glycoproteins and glycolipids. A4GALT, or alpha-1,4-galactosyltransferase, is crucial in the synthesis of glycan structures that contribute to cellular communication, recognition, and immune response. Alterations or deficiencies in A4GALT can lead to various pathological conditions, including immune deficiencies and certain types of cancer, highlighting the importance of this enzyme in maintaining cellular homeostasis. Research into A4GALT recombinant protein aims to elucidate its biochemical properties, substrate specificity, and regulation mechanisms, which can further our understanding of glycosylation pathways. This knowledge not only contributes to basic biological science but also holds potential for therapeutic applications, such as the development of targeted treatments for conditions arising from glycosylation defects. Additionally, the production of A4GALT as a recombinant protein facilitates the exploration of its function in vivo, enabling scientists to study its interactions within complex biological systems. Overall, investigating A4GALT offers critical insights into the broader implications of glycosylation in health and disease, paving the way for novel intervention strategies.
