Analytical Data
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基因名
COL4a4
- Application
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别名
COL4a4；Collagen alpha-4(IV) chain
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P02462
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表达区间
30-167aa
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氨基酸序列
GCAGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEGPQGPPGQKGDTGEPGLPGTKGTRGPPGASGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGLPGFAGNPGPPGLPGMKGDPGEILGHVP
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分子量
39.9kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
COL4A4, a gene encoding the alpha 4 chain of type IV collagen, plays a crucial role in the structural integrity of basement membranes throughout the body, particularly in the kidneys, eyes, and ears. Mutations in COL4A4 are linked to various inherited diseases, such as Alport syndrome, which is characterized by progressive renal failure, hearing loss, and ocular abnormalities due to defects in the type IV collagen network. The study of COL4A4 recombinant proteins is pivotal for understanding the molecular mechanisms underlying these disorders, as well as for the development of potential therapeutic strategies. By producing and characterizing recombinant COL4A4 proteins, researchers can investigate the impact of specific mutations on protein structure and function, elucidate the pathophysiological pathways involved in related diseases, and explore the potential for gene therapy or protein replacement therapies. Furthermore, the study of COL4A4 recombinant proteins could facilitate the development of biomarkers for early diagnosis and monitoring of related conditions, highlighting its significance in both clinical and research contexts.
