Analytical Data
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Gene name
TIPARP
- Application
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Alternative Names
TIPARP；PARP7；Protein mono-ADP-ribosyltransferase TIPARP
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
TIPARP
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Expression Region
1-657aa
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AA Sequence
MEMETTEPEPDCVVQPPSPPDDFSCQMRLSEKITPLKTCFKKKDQKRLGTGTLRSLRPILNTLLESGSLDGVFRSRNQSTDENSLHEPMMKKAMEINSSCPPAENNMSVLIPDRTNVGDQIPEAHPSTEAPERVVPIQDHSFPSETLSGTVADSTPAHFQTDLLHPVSSDVPTSPDCLDKVIDYVPGIFQENSFTIQYILDTSDKLSTELFQDKSEEASLDLVFELVNQLQYHTHQENGIEICMDFLQGTCIYGRDCLKHHTVLPYHWQIKRTTTQKWQSVFNDSQEHLERFYCNPENDRMRMKYGGQEFWADLNAMNVYETTEFDQLRRLSTPPSSNVNSIYHTVWKFFCRDHFGWREYPESVIRLIEEANSRGLKEVRFMMWNNHYILHNSFFRREIKRRPLFRSCFILLPYLQTLGGVPTQAPPPLEATSSSQIICPDGVTSANFYPETWVYMHPSQDFIQVPVSAEDKSYRIIYNLFHKTVPEFKYRILQILRVQNQFLWEKYKRKKEYMNRKMFGRDRIINERHLFHGTSQDVVDGICKHNFDPRVCGKHATMFGQGSYFAKKASYSHNFSKKSSKGVHFMFLAKVLTGRYTMGSHGMRRPPPVNPGSVTSDLYDSCVDNFFEPQIFVIFNDDQSYPYFVIQYEEVSNTVSI
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Molecular Weight
82.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TIPARP (TCDD-Inducible Poly(ADP-ribose) Polymerase) is a member of the poly(ADP-ribose) polymerase (PARP) family, which plays a crucial role in cellular responses to DNA damage and stress. The enzyme was first identified due to its inducibility by toxic environmental pollutants, particularly dioxins, which are known to interfere with cellular signaling and homeostasis. Research has revealed that TIPARP is involved in various cellular processes, including gene regulation, cellular stress response, and inflammation. Its ability to modify proteins through ADP-ribosylation suggests a pivotal role in modulating signaling pathways pertinent to tumorigenesis and autoimmune diseases. Furthermore, TIPARP has been shown to exhibit anti-apoptotic effects, which may have implications for cancer therapy and understanding the mechanisms of resistance to chemotherapy. The growing interest in TIPARP as a potential therapeutic target has led to the exploration of its structural properties, functional mechanisms, and interactions with other cellular molecules, positioning it as a critical focus in the field of molecular biology and pharmacology. Researchers are particularly interested in the development of TIPARP inhibitors, which could pave the way for novel treatment strategies in cancer and other diseases linked to dysregulated ADP-ribosylation. As the understanding of TIPARP's role expands, its significance in health and disease continues to motivate further investigation into its potential applications in precision medicine.
