Analytical Data
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Gene name
mrkD
- Application
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Alternative Names
mrkD;Fimbria adhesin Protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P21648
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Expression Region
19-321aa
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AA Sequence
WASCWQSNSAYEINMAMGRVVVSPDLPVGSVIATKTWTMPDNNTIYVTCDRNTTLKSDAKVVAAGLVQGANKVYSTAIPGIGLRFSRKGAISMIYPDSYTTTGSSFRLVGSTFTLDIIKTSTTTGSGTLASGPYTEYGPGFTILKTSLNADAITIVSPSCTILGGKNMNVDIGTIKRADLKGVGTWAGGTPFDIKLECSGGVSVSGYANINTSFSGTLATNTSANQGVLLNEKTGNSAAKGVGVQVIKDNTPLEFNKKHNIGTLQSQETRYITLPLHARFYQYAPTTSTGEVESHLVFNLTYD
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Molecular Weight
39.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Recombinant proteins play a significant role in various fields, including biotechnology, medicine, and research. One such protein is mrkD, which is a key component of the fimbrial adhesin system found in certain bacteria, particularly those belonging to the Escherichia coli group. The mrkD protein is responsible for mediating bacterial adhesion to host tissues, which is a critical step in the establishment of infections. Understanding the structure and function of mrkD can provide insights into the mechanisms of bacterial pathogenesis and host interactions. Moreover, exploring the properties of mrkD could facilitate the development of novel therapeutic strategies and vaccines aimed at preventing infections caused by these pathogenic bacteria. Current research focuses on the cloning, expression, and purification of the mrkD recombinant protein, utilizing advanced techniques such as molecular cloning and protein engineering. Characterizing mrkD’s interaction with host cells and its role in biofilm formation is essential for elucidating how bacteria resist the host immune response and persist in various environments. The findings from these studies can also contribute to the design of anti-adhesive therapies that inhibit bacterial colonization, thereby mitigating infection rates and improving patient outcomes. Overall, the investigation of mrkD recombinant protein not only enhances our molecular understanding of bacterial adhesion but also aids in the innovation of cutting-edge solutions to combat bacterial infections.











