Analytical Data
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基因名
NELFE
- Application
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别名
NELFE;RD;RDBP;Negative elongation factor E
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P18615
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表达区间
1-380aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMLVIPPGLSEEEEALQKKFNKLKKKKKALL ALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAE TKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRK SLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSA SPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRER DRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAF SPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIA RKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF
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分子量
45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NELFE (Negative Elongation Factor E) is a multi-subunit protein complex that plays a significant role in regulating transcription elongation by RNA polymerase II. Research into NELFE has gained momentum due to its critical involvement in various cellular processes, including transcription regulation, RNA processing, and co-transcriptional events. Dysregulation of NELFE function has been implicated in several diseases, including cancer and neurological disorders, highlighting the importance of understanding its molecular mechanisms. Studies have shown that NELFE interacts with other transcription factors and interacts with RNA to modulate gene expression, thereby influencing cell proliferation and differentiation. The characterization and reconstitution of NELFE in vitro are essential for elucidating its structure-function relationships and its dynamics during transcription. By exploring NELFE's interactions and regulatory mechanisms, researchers aim to uncover potential therapeutic targets and strategies to manipulate transcriptional control pathways in disease contexts, making the study of NELFE a pivotal focus in molecular biology and translational research.
