Analytical Data
-
Gene name
NDUFAF4
- Application
-
Alternative Names
NDUFAF4;C6orf66;HRPAP20;NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9P032
-
Expression Region
1-175aa
-
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGALVIR GIRNFNLENR AEREISKMKP SVAPRHPSTN SLLREQISLY PEVKGEIARK DEKLLSFLKD VYVDSKDPVS SLQVKAAETC QEPKEFRLPK DHHFDMINIK SIPKGKISIV EALTLLNNHK LFPETWTAEK IMQEYQLEQK DVNSLLKYFV TFEVEIFPPE DKKAIRSK
-
Molecular Weight
23 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NDUFAF4 (NADH:ubiquinone oxidoreductase assembly factor 4) is a crucial protein involved in the assembly and maintenance of the mitochondrial NADH dehydrogenase (complex I) of the electron transport chain. Research has shown that NDUFAF4 plays a significant role in the proper folding and integration of complex I subunits, thereby influencing mitochondrial function and energy production. Mutations or dysfunction in NDUFAF4 have been associated with various mitochondrial diseases, highlighting its importance in cellular metabolism and bioenergetics. As mitochondrial dysfunction is linked to aging and a range of neurodegenerative disorders, understanding NDUFAF4's mechanisms can provide insights into potential therapeutic strategies. Recent studies have utilized recombinant protein technologies to express and purify NDUFAF4, enabling detailed investigations into its structural properties and interaction with other complex I components. This research is integral to deciphering the molecular pathways underlying mitochondrial assembly and function, as well as the implications of NDUFAF4 in both health and disease. By exploring NDUFAF4 through recombinant approaches, scientists aim to elucidate its role in mitochondrial integrity and identify possible interventions for mitochondrial-related conditions.
