Analytical Data
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基因名
NDUFAF1
- Application
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别名
NDUFAF1;CIA30;Complex I intermediate-associated Protein 30. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y375
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表达区间
25-327aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSYPFLGIR FAEYSSSLQK PVASPGKASS QRKTEGDLQG DHQKEVALDI TSSEEKPDVS FDKAIRDEAI YHFRLLKDEI VDHWRGPEGH PLHEVLLEQA KVVWQFRGKE DLDKWTVTSD KTIGGRSEVF LKMGKNNQSA LLYGTLSSEA PQDGESTRSG YCAMISRIPR GAFERKMSYD WSQFNTLYLR VRGDGRPWMV NIKEDTDFFQ RTNQMYSYFM FTRGGPYWQE VKIPFSKFFF SNRGRIRDVQ HELPLDKISS IGFTLADKVD GPFFLEIDFI GVFTDPAHTE EFAYENSPEL NPRLFK
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分子量
37 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDUFAF1, or NADH:ubiquinone oxidoreductase assembly factor 1, is a critical protein involved in the assembly of complex I, a key component of the mitochondrial respiratory chain. Proper functioning of complex I is essential for cellular energy production, as it plays a pivotal role in oxidative phosphorylation. Mutations or dysfunctions in NDUFAF1 have been implicated in various mitochondrial diseases, leading to profound energy deficits and a range of metabolic disorders. The study of NDUFAF1-recombinant proteins aims to elucidate the mechanisms underlying complex I assembly and function, as well as to explore the pathophysiological consequences of NDUFAF1 deficiency. Additionally, research into NDUFAF1 can shed light on potential therapeutic strategies for mitochondrial diseases and offer insights into the broader implications of mitochondrial dysfunction in aging and neurodegenerative disorders. By employing recombinant protein techniques, scientists can produce NDUFAF1 in a controlled laboratory setting, allowing for detailed biochemical analyses, structural characterization, and functional assays. This research not only deepens our understanding of mitochondrial biology but also holds promise for developing interventions aimed at restoring mitochondrial function in affected individuals. Overall, NDUFAF1 and its recombinant protein studies are fundamental to advancing our knowledge of mitochondrial integrity and therapeutic approaches for associated diseases.
