Analytical Data
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Gene name
NAE1
- Application
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Alternative Names
NAE1;APPBP1;NEDD8-activating enzyme E1 regulatory subunit
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13564
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Expression Region
1-534aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVP LRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAY WIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIV AVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIA FYSYTFS
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Molecular Weight
63 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The NAE1 (NEDD8-activating enzyme 1) recombinant protein has emerged as a significant focus of research due to its pivotal role in the ubiquitin-like modification process. NAE1 is an essential component of the NEDD8 conjugation pathway, which is crucial for regulating various cellular processes, including cell cycle progression, apoptosis, and signal transduction. Dysregulation of NAE1 and the NEDD8 pathway has been implicated in several pathologies, particularly cancer, where it contributes to tumor growth and resistance to therapy. The interest in NAE1 recombinant proteins stems from their potential as therapeutic targets; thus, understanding their structure and function can provide insights for the development of small-molecule inhibitors. Furthermore, NAE1’s involvement in the modulation of protein stability and activity highlights its relevance in the broader context of proteostasis and cellular homeostasis. Consequently, ongoing studies of NAE1 recombinant proteins aim to explore their biochemical properties, interaction networks, and the possibility of leveraging them in drug discovery and therapeutic interventions against malignancies and other diseases associated with aberrant NEDD8 signaling.
