Analytical Data
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基因名
CYP21A2
- Application
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别名
CYP21A2；CYP21；CYP21B；Steroid 21-hydroxylase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P08686
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表达区间
1-494aa
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氨基酸序列
MLLLGLLLLPLLAGARLLWNWWKLRSLHLPPLAPGFLHLLQPDLPIYLLG LTQKFGPIYRLHLGLQDVVVLNSKRTIEEAMVKKWADFAGRPEPLTYKLV SKNYPDLSLGDYSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCERM RAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKT WSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVA GQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTL SWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIA EVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWER PHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFT LLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQNQ
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分子量
58 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CYP21A2, the gene encoding the enzyme steroid 21-hydroxylase, plays a crucial role in steroidogenesis by catalyzing the conversion of progesterone and 17-hydroxyprogesterone into 11-deoxycorticosterone and 11-deoxycortisol, respectively. Deficiencies in CYP21A2 result in congenital adrenal hyperplasia (CAH), a condition characterized by impaired adrenal steroid production, leading to excess androgens and various clinical manifestations, including adrenal crisis and disorders of sexual development. The study of CYP21A2 recombinant protein has gained significant importance in understanding the pathophysiology of CAH and allows for the exploration of genotype-phenotype correlations. By producing and characterizing recombinant CYP21A2, researchers can investigate the enzymatic activity, stability, and inhibition of the enzyme, providing insights into potential therapeutic strategies. In addition, such studies facilitate the development of diagnostic tools for more accurate detection of CYP21A2 mutations in affected individuals. Enhanced knowledge of CYP21A2 biochemistry not only aids in better understanding individual variations in enzyme activity but also contributes to advancing targeted gene therapy and personalized medicine approaches for CAH management. Overall, the research on CYP21A2 recombinant proteins is integral to unraveling the complexities of steroid metabolism and improving clinical outcomes for patients with adrenal disorders.
