Analytical Data
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基因名
LIN28B
- Application
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别名
CSDD 2; CSDD2 ; FLJ16517; Lin 28 homolog B (C. elegans); Lin 28.2; Lin-28B; LIN28B; LN28B_HUMAN; Protein lin-28 homolog B
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6ZN17
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表达区间
1-250aa
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氨基酸序列
MAEGGASKGGGEEPGKLPEPAEEESQVLRGTGHCKWFNVRMGFGFISMINREGSPLDIPVDVFVHQSKLFMEGFRSLKEGEPVEFTFKKSSKGLESIRVTGPGGSPCLGSERRPKGKTLQKRKPKGDRCYNCGGLDHHAKECSLPPQPKKCHYCQSIMHMVANCPHKNVAQPPASSQGRQEAESQPCTSTLPREVGGGHGCTSPPFPQEARAEISERSGRSPQEASSTKSSIAPEEQSKKGPSVQKRKKT
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分子量
53.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LIN28B is a member of the LIN28 family of RNA-binding proteins that play a crucial role in post-transcriptional regulation of gene expression. Originally identified for its involvement in stem cell maintenance and pluripotency, LIN28B has garnered significant attention due to its implications in cancer biology and development. The protein is known to bind to specific miRNAs, particularly let-7, thereby inhibiting their maturation and leading to the upregulation of oncogenes associated with various malignancies. The study of LIN28B and its recombinant protein form has expanded to investigate its functional roles in cellular processes such as differentiation, metabolism, and immune responses. Additionally, lin28b expression often correlates with poor prognosis in several cancers, making it a potential biomarker and therapeutic target. Researchers are increasingly focused on understanding the molecular mechanisms underlying LIN28B's activity and its potential applications in regenerative medicine and cancer therapy. Through advanced techniques such as protein engineering, CRISPR-Cas9 gene editing, and high-throughput screening, scientists aim to elucidate the pathways regulated by LIN28B and explore its potential for novel therapeutic interventions. The ongoing research into LIN28B recombinant protein not only enhances our understanding of stem cell biology and tumorigenesis but also holds promise for the development of innovative strategies for disease management and treatment.












