Analytical Data
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Gene name
LIMS3
- Application
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Alternative Names
LIMS3; PINCH3LIM and senescent cell antigen-like-containing domain protein 3; Particularly interesting new Cys-His protein 3; PINCH-3
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0CW19
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Expression Region
1-117aa
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AA Sequence
MAFSGRARPCIIPENEEIPRAALNTVHEANGTEDERAVSKLQRRHSDVKVYKEFCDFYAKFNMANALASATCERCKGGFAPAETIVNSNGELYHEQCFVCAQCFQQFPEGLFYEERT
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Molecular Weight
12.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LIMS3, or LIM domain containing protein 3, is a member of the LIM protein family, which is known for its role in various cellular processes, including cell differentiation, proliferation, and signaling pathways. Research has increasingly focused on LIMS3 due to its potential implications in developmental biology and disease states, particularly in cancer. The expression of LIMS3 is tissue-specific, with significant roles identified in muscle and neuronal tissues, suggesting its involvement in muscle and nerve development. Additionally, dysregulation of LIMS3 has been linked to tumorigenesis, highlighting its relevance in cancer research. Recombinant protein studies of LIMS3 are crucial for elucidating its structural and functional properties, as well as for understanding its interactions with other cellular components. Such studies may also enable the identification of potential therapeutic targets, paving the way for novel interventions in diseases where LIMS3 is implicated. Overall, the exploration of LIMS3 as a recombinant protein holds promise for advancing our comprehension of its biological functions and therapeutic applications, making it a significant focus in molecular biology and biomedical research.
