Analytical Data
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Gene name
GUCY1b3
- Application
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Alternative Names
GUCY1b3；GUC1B3；GUCSB3；GUCY1B3；Guanylate cyclase soluble subunit beta-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q02153
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Expression Region
1-619aa
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AA Sequence
MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETKQDDD
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Molecular Weight
70.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GUCY1B3, a gene encoding the β3 subunit of soluble guanylate cyclase (sGC), plays a crucial role in the nitric oxide (NO) signaling pathway, which is essential for various physiological processes, including vasodilation, neurotransmission, and cellular signaling. Mutations in GUCY1B3 have been linked to inherited retinal diseases, particularly in patients with retinitis pigmentosa and Leber's congenital amaurosis, leading to significant vision loss. The recombinant protein of GUCY1B3 provides a valuable tool for understanding the functional properties of sGC, as well as the pathophysiological mechanisms arising from GUCY1B3 mutations. By studying the recombinant protein, researchers can investigate its interaction with NO and other signaling molecules, the structural basis of its activity, and the downstream effects on cGMP production. This knowledge can pave the way for potential therapeutic strategies targeting sGC to treat related diseases. Moreover, the generation of GUCY1B3 recombinant protein can facilitate high-throughput screening for small molecules that may enhance or inhibit its activity, thus offering insights into drug discovery aimed at correcting the functional deficits caused by specific mutations. Overall, the study of GUCY1B3 recombinant protein holds promise not only for basic research into guanylate cyclase function but also for translational applications in addressing genetic disorders linked to this critical signaling pathway.
