Analytical Data
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Gene name
ADRb1
- Application
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Alternative Names
ADRb1；ADRB1R；B1AR；Beta-1 adrenergic receptor
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P08588
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Expression Region
1-477aa
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AA Sequence
MGAGVLVLGASEPGNLSSAAPLPDGAATAARLLVPASPPASLLPPASESPEPLSQQWTAGMGLLMALIVLLIVAGNVLVIVAIAKTPRLQTLTNLFIMSLASADLVMGLLVVPFGATIVVWGRWEYGSFFCELWTSVDVLCVTASIETLCVIALDRYLAITSPFRYQSLLTRARARGLVCTVWAISALVSFLPILMHWWRAESDEARRCYNDPKCCDFVTNRAYAIASSVVSFYVPLCIMAFVYLRVFREAQKQVKKIDSCERRFLGGPARPPSPSPSPVPAPAPPPGPPRPAAAAATAPLANGRAGKRRPSRLVALREQKALKTLGIIMGVFTLCWLPFFLANVVKAFHRELVPDRLFVFFNWLGYANSAFNPIIYCRSPDFRKAFQRLLCCARRAARRRHATHGDRPRASGCLARPGPPPSPGAASDDDDDDVVGATPPARLLEPWAGCNGGAAADSDSSLDEPCRPGFASESKV
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Molecular Weight
52.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The ADRB1 (adrenergic receptor beta 1) protein is a key player in the adrenergic signaling pathway, primarily involved in the regulation of cardiovascular function, including heart rate and contractility. It belongs to the G protein-coupled receptor (GPCR) family and is widely studied due to its significant role in pharmacology and medicine, particularly concerning heart diseases, hypertension, and heart failure. The interaction of ADRB1 with catecholamines, such as adrenaline and norepinephrine, triggers various intracellular signaling cascades that ultimately influence cardiac output and vascular tone. Research on recombinant ADRB1 protein has gained traction as it allows for the detailed study of receptor structure, function, and pharmacology in a controlled environment. Advances in recombinant DNA technology enable the production of large quantities of purified ADRB1 protein, facilitating its use in binding assays, pharmacological screening, and structural studies using techniques like X-ray crystallography and cryo-electron microscopy. Understanding the detailed mechanisms of ADRB1 signaling can lead to the development of new therapeutic strategies and drugs aimed at modulating its activity for various clinical conditions. The refinement of recombinant expression systems also paves the way for novel studies on receptor mutations and their implications in disease, further underscoring ADRB1's relevance in biomedical research.
