Analytical Data
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Gene name
PYR1
- Application
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Alternative Names
PYR1；Multifunctional Protein CAD
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O49686
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Expression Region
1-191aa
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AA Sequence
MPSELTPEERSELKNSIAEFHTYQLDPGSCSSLHAQRIHAPPELVWSIVRRFDKPQTYKHFIKSCSVEQNFEMRVGCTRDVIVISGLPANTSTERLDILDDERRVTGFSIIGGEHRLTNYKSVTTVHRFEKENRIWTVVLESYVVDMPEGNSEDDTRMFADTVVKLNLQKLATVAEAMARNSGDGSGSQVT
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Molecular Weight
69.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PYR1, a member of the PYR/PYL (PYRABACTIN RESISTANCE 1/PYRABACTIN RESISTANCE-LIKE) family of receptors, plays a critical role in plants' response to abscisic acid (ABA), a key hormone that regulates various physiological processes including seed germination, stomatal closure, and stress responses. Understanding the structure and function of PYR1 is essential for deciphering the ABA signaling pathway, which is crucial for plant adaptation to environmental stresses such as drought and salinity. Research on PYR1 has revealed its ability to bind ABA and subsequently interact with protein phosphatases, specifically PP2Cs (protein phosphatase 2Cs), to inhibit their activity and thus activate downstream signaling. This interaction serves as a pivotal mechanism through which ABA modulates gene expression and physiological responses. Investigating the nuances of PYR1 function, including its structural dynamics and interaction with other signaling components, offers insights into engineering crops with enhanced stress tolerance, potentially leading to improved agricultural resilience in the face of climate change. Recent advancements in recombinant protein technology have enabled the production of purified PYR1, facilitating more in-depth biochemical and biophysical studies. These investigations aim to elucidate the molecular mechanisms underpinning PYR1's role in ABA signaling, thereby advancing our understanding of plant hormone signaling networks and their applications in crop improvement strategies.
