Analytical Data
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基因名
NAD
- Application
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别名
NAD；SIR2L；SIR2L2；NAD-dependent Protein deacetylase sirtuin-2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8IXJ6
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表达区间
2-389aa
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氨基酸序列
AEPDPSHPL ETQAGKVQEA QDSDSDSEGG AAGGEADMDF LRNLFSQTLS LGSQKERLLD ELTLEGVARY MQSERCRRVI CLVGAGISTS AGIPDFRSPS TGLYDNLEKY HLPYPEAIFE ISYFKKHPEP FFALAKELYP GQFKPTICHY FMRLLKDKGL LLRCYTQNID TLERIAGLEQ EDLVEAHGTF YTSHCVSASC RHEYPLSWMK EKIFSEVTPK CEDCQSLVKP DIVFFGESLP ARFFSCMQSD FLKVDLLLVM GTSLQVQPFA SLISKAPLST PRLLINKEKA GQSDPFLGMI MGLGGGMDFD SKKAYRDVAW LGECDQGCLA LAELLGWKKE LEDLVRREHA SIDAQSGAGV PNPSTSASPK KSPPPAKDEA RTTEREKPQ
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分子量
43.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NAD (Nicotinamide Adenine Dinucleotide) is a crucial coenzyme found in all living cells, playing a key role in various metabolic processes, including cellular respiration and energy production. Recent studies have emphasized the significance of NAD in maintaining cellular homeostasis and its involvement in redox reactions. The research on NAD and its reconstituted proteins has gained momentum due to the emerging evidence that NAD is not only pivotal in energy metabolism but also in regulating cellular aging and stress resistance. NAD-dependent enzymes, such as sirtuins and poly(ADP-ribose) polymerases (PARPs), have become focal points of study, linking NAD levels to aging, neurodegenerative diseases, and metabolic disorders. Understanding the reconstitution of NAD-dependent proteins aids in unraveling their biological functions and can offer potential therapeutic strategies for age-related conditions and metabolic diseases. Over the past few years, advances in protein engineering and synthetic biology have enabled researchers to create NAD-reconstituted proteins with tailored functionalities, paving the way for innovative applications in biotechnology and medicine. This growing body of research underscores the importance of NAD in both fundamental biology and therapeutic innovation, highlighting a promising avenue for future scientific exploration.
