Analytical Data
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基因名
KIF12
- Application
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别名
Kif12; KIF12_HUMAN; Kinesin family member 12 ; Kinesin-like protein KIF12; OTTHUMP00000021965; RP11-56P10.3
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96FN5
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表达区间
1-646aa
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氨基酸序列
MEERGSPDGD LARSLEQGPE GPETPIQVVL RVRPMSAAEL RRGQQSVLHC SGTRTLQGGP EVAFRFGAVL DAARTQEDVF RACGVRRLGE LALRGFSCTV FTFGQTGSGK TYTLTGPPPQ GEGVPVPPSL AGIMQRTFAW LLDRVQHLGA PVTLRASYLE IYNEQVRDLL SLGSPRPLPV RWNKTRGFYV EQLRVVEFGS LEALMELLQT GLSRRRNSAH TLNQASSRSH ALLTLYISRQ TAQQMPSVDP GEPPVGGKLC FVDLAGSEKV AATGSRGELM LEANSINRSL LALGHCISLL LDPQRKQSHI PFRDSKLTKL LADSLGGRGV TLMVACVSPS AQCLPETLST LRYASRAQRV TTRPQAPKSP VAKQPQRLET EMLQLQEENR RLQFQLDQMD CKASGLSGAR VAWAQRNLYG MLQEFMLENE RLRKEKSQLQ NSRDLAQNEQ RILAQQVHAL ERRLLSACYH HQQGPGLTPP CPCLMAPAPP CHALPPLYSC PCCHICPLCR VPLAHWACLP GEHHLPQVLD PEASGGRPPS ARPPPWAPPC SPGSAKCPRE RSHSDWTQTR VLAEMLTEEE VVPSAPPLPV RPPKTSPGLR GGAGVPNLAQ RLEALRDQIG SSLRRGRSQP PCSEGARSPG QVLPPH
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分子量
70.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KIF12, or kinesin family member 12, is a motor protein that plays a crucial role in intracellular transport along microtubules, which are essential components of the cytoskeleton in eukaryotic cells. It is part of the kinesin superfamily, a group of proteins that utilize ATP hydrolysis to move cargoes, such as organelles and protein complexes, within cells. Research has indicated that KIF12 may be involved in critical cellular processes, including cell division and migration, making it a potential contributor to developmental biology and cancer research. Mutations or dysregulation of KIF12 have been associated with various diseases, highlighting its importance in maintaining cellular homeostasis. Given its functional relevance, the study of KIF12 recombinant protein allows researchers to explore its structure-function relationship, investigate its biochemical properties, and assess its role in cellular dynamics. By producing and purifying KIF12 in a controlled environment, scientists can conduct in vitro assays to decipher its motor activity, investigate interaction with various cargoes, and evaluate its potential as a target for therapeutic intervention in diseases related to cytoskeletal dysfunction. Overall, research on KIF12 recombinant protein encompasses a multidisciplinary approach, blending molecular biology, biochemistry, and cellular biology, thus contributing to the broader understanding of cellular mechanisms and potential avenues for medical advancements.
