Cat: PA1000-1757

Recombinant Human KRT14 Protein,His

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Analytical Data

  • Gene name

    KRT14

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    KRT14;Keratin. type I cytoskeletal 14

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P02533

  • Expression Region

    1-472aa

  • AA Sequence

    MGSSHHHHHH SSGLVPRGSH MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIK DYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQN LEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN

  • Molecular Weight

    54 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

Keratin 14 (KRT14) is a type of intermediate filament protein primarily expressed in the basal layer of stratified epithelial tissues, including the skin and mucous membranes. Its critical role in providing structural integrity and mechanical resilience has made it a focal point of research, especially in understanding skin diseases and disorders. Mutations in the KRT14 gene are associated with various keratinization disorders, such as epidermolysis bullosa simplex, which is characterized by skin fragility and blister formation. The recombinant expression of KRT14 protein serves as a valuable tool for studying its biochemical properties and interactions in vitro. Additionally, it aids in the development of potential therapeutic strategies, including gene therapy and tissue engineering, to treat skin-related conditions. By generating recombinant KRT14, researchers can investigate its role in cellular mechanisms, adhesion, and signaling pathways, thereby contributing to the overall understanding of epithelial biology and advancing the field of regenerative medicine. Thus, the study of KRT14 recombinant protein is pivotal not only for elucidating the molecular underpinnings of skin diseases but also for paving the way towards innovative treatment modalities.

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