Analytical Data
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基因名
TCF7
- Application
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别名
TCF7；TCF1；Transcription factor 7
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P36402
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表达区间
1-384aa
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氨基酸序列
MPQLDSGGGGAGGGDDLGAPDELLAFQDEGEEQDDKSRDSAAGPERDLAELKSSLVNESEGAAGGAGIPGVPGAGAGARGEAEALGREHAAQRLFPDKLPEPLEDGLKAPECTSGMYKETVYSAFNLLMHYPPPSGAGQHPQPQPPLHKANQPPHGVPQLSLYEHFNSPHPTPAPADISQKQVHRPLQTPDLSGFYSLTSGSMGQLPHTVSWFTHPSLMLGSGVPGHPAAIPHPAIVPPSGKQELQPFDRNLKTQAESKAEKEAKKPTIKKPLNAFMLYMKEMRAKVIAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKRRSREKHQESTTETNWPRELKDGNGQESLSMSSSSSPA
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分子量
49.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TCF7, a member of the T-cell factor/lymphoid enhancer factor (TCF/LEF) family of transcription factors, plays a critical role in the Wnt signaling pathway, which is essential for various developmental processes and cell fate decisions. It is involved in the regulation of gene expression during embryonic development, stem cell maintenance, and differentiation. Dysregulation of TCF7 has been implicated in multiple diseases, including various types of cancer, where aberrant Wnt signaling can lead to uncontrolled cell proliferation and survival. Due to its central function in these biological processes, TCF7 has garnered attention as a potential therapeutic target. Researchers are interested in understanding the structural and functional dynamics of TCF7 through the development of recombinant TCF7 proteins, enabling the study of their interactions with Wnt ligands, co-factors, and target DNA sequences. Furthermore, the production of recombinant TCF7 proteins allows for in vitro assays to elucidate the mechanistic details of Wnt signaling and its impact on cellular behaviors. This research not only contributes to the fundamental understanding of TCF7's role in cell biology but also holds promise for the development of novel strategies for therapeutic intervention in Wnt-related diseases.
