Analytical Data
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基因名
CD40
- Application
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别名
CD40;TNFRSF5;Tumor necrosis factor receptor superfamily member 5
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P25942
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表达区间
21-193aa
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氨基酸序列
EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDT WNRETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCV LHRSCSPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETK DLVVQQAGTNKTDVVCGPQDRLR
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分子量
45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD40 is a co-stimulatory molecule that plays a crucial role in the regulation of immune responses, particularly in the interactions between antigen-presenting cells (APCs) and T cells. The CD40-CD40L (CD154) interaction is vital for the activation of B cells, enhancing antibody production, and facilitating the development of germinal centers, which are essential for generating high-affinity antibodies. Dysregulation of CD40 signaling has been implicated in various autoimmune diseases, cancers, and infections, making it a significant target for therapeutic interventions. The recombinant protein form of CD40 can be used as a tool to study its functional properties, stability, and potential applications in vaccine development and immunotherapy. By expressing CD40 in a recombinant system, researchers can investigate its role in T cell activation, the modulation of innate immune responses, and the potential for enhancing the efficacy of cancer vaccines. Furthermore, understanding the molecular mechanisms of CD40 signaling can lead to the development of novel therapeutic strategies aimed at modulating immune responses in various disease contexts. The exploration of CD40's role through its recombinant form yields valuable insights that could pave the way for advancements in immunotherapeutic techniques, aiming to harness or regulate the immune system for improved outcomes in disease treatment and prevention.












