Analytical Data
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Gene name
KCNJ4
- Application
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Alternative Names
KCNJ4; IRK3; Inward rectifier potassium channel 4; HIRK2; HRK1; Hippocampal inward rectifier; HIR; Inward rectifier K(+ channel Kir2.3; IRK-3; Potassium channel. inwardly rectifying subfamily J member 4
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48050
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Expression Region
1-445aa
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AA Sequence
MHGHSRNGQAHVPRRKRRNRFVKKNGQCNVYFANLSNKSQRYMADIFTTCVDTRWRYMLMIFSAAFLVSWLFFGLLFWCIAFFHGDLEASPGVPAAGGPAAGGGGAAPVAPKPCIMHVNGFLGAFLFSVETQTTIGYGFRCVTEECPLAVIAVVVQSIVGCVIDSFMIGTIMAKMARPKKRAQTLLFSHHAVISVRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPYMTQEGEYLPLDQRDLNVGYDIGLDRIFLVSPIIIVHEIDEDSPLYGMGKEELESEDFEIVVILEGMVEATAMTTQARSSYLASEILWGHRFEPVVFEEKSHYKVDYSRFHKTYEVAGTPCCSARELQESKITVLPAPPPPPSAFCYENELALMSQEEEEMEEEAAAAAAVAAGLGLEAGSKEEAGIIRMLEFGSHLDLERMQASLPLDNISYRRESAI
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Molecular Weight
75.35 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KCNJ4, also known as the inwardly rectifying potassium channel 4, is a member of the KCNJ family, which plays a crucial role in regulating cellular excitability and maintaining ion balance in various tissues. This protein is primarily expressed in the heart and brain, where it contributes to the stabilization of resting membrane potential and the modulation of action potentials. Research on KCNJ4 has gained significance due to its involvement in various physiological processes and potential pathological conditions. Alterations in KCNJ4 function have been linked to cardiac arrhythmias, neurological disorders, and other diseases, highlighting its importance in cellular function and signaling pathways. Recombinant KCNJ4 protein expression systems have been developed to study its biophysical properties, pharmacological profiles, and interactions with other cellular proteins. Understanding the structure-function relationship of KCNJ4 through recombinant protein studies can provide insights into the mechanisms underlying its role in health and disease, paving the way for the development of novel therapeutic strategies targeting potassium channels to treat arrhythmias and other related conditions. Additionally, KCNJ4’s role in drug interactions and its potential as a biomarker for certain diseases further emphasizes the necessity for continued research in this area to explore its full therapeutic potential.
