Analytical Data
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基因名
NFYC
- Application
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别名
NFYC；Nuclear transcription factor Y subunit gamma
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13952
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表达区间
1-458aa
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氨基酸序列
MSTEGGFGGT SSSDAQQSLQ SFWPRVMEEI RNLTVKDFRV QELPLARIKK IMKLDEDVKM ISAEAPVLFA KAAQIFITEL TLRAWIHTED NKRRTLQRND IAMAITKFDQ FDFLIDIVPR DELKPPKRQE EVRQSVTPAE PVQYYFTLAQ QPTAVQVQGQ QQGQQTTSST TTIQPGQIII AQPQQGQTTP VTMQVGEGQQ VQIVQAQPQG QAQQAQSGTG QTMQVMQQII TNTGEIQQIP VQLNAGQLQY IRLAQPVSGT QVVQGQIQTL ATNAQQGQRN ASQGKPRRCL KETLQITQTE VQQGQQQFSQ FTDGQRNSVQ QARVSELTGE AEPREVKATG NSTPCTSSLP TTHPPSHRAG ASCVCCSQPQ QSSTSPPPSD ALQWVVVEVS GTPNQLETHR ELHAPLPGMT SLSPLHPSQQ LYQIQQVTMP AGQDLAQPMF IQSANQPSDG QAPQVTGD
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分子量
50.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NFYC (Nuclear Transcription Factor Y Subunit C) is a key protein involved in the regulation of various gene expression processes crucial for plant growth and development, as well as responses to environmental stimuli. The NFYC gene family is highly conserved across different species, highlighting its fundamental role in cellular functions. Recent studies have revealed NFYC's involvement in numerous physiological and developmental processes, including flowering time regulation, stress response, and hormone signaling pathways. The protein functions by forming heterotrimeric complexes with NFYA and NFYB subunits, binding to CCAAT boxes in the promoter regions of various target genes, thus influencing transcriptional activity. Given its pivotal role in plant biology, research on NFYC has garnered interest not only to understand the mechanisms of transcription regulation but also to explore its potential applications in crop improvement and biotechnology. Advances in molecular techniques, such as CRISPR/Cas9 gene editing and transcriptomics, have opened new avenues for investigating NFYC's functional roles. As climate change intensifies the challenges faced by agriculture, understanding NFYC's mechanisms could facilitate the development of stress-resistant crops, ultimately contributing to food security and sustainable agricultural practices. Therefore, studying NFYC and its associated pathways offers significant potential for enhancing our understanding of plant biology and addressing global agricultural challenges.
