Cat: PA2000-303DB

Recombinant Human NFYC Protein,His

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Analytical Data

  • Gene name

    NFYC

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    NFYC;Nuclear transcription factor Y subunit gamma

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q13952

  • Expression Region

    1-458aa

  • AA Sequence

    MSTEGGFGGT SSSDAQQSLQ SFWPRVMEEI RNLTVKDFRV QELPLARIKK IMKLDEDVKM ISAEAPVLFA KAAQIFITEL TLRAWIHTED NKRRTLQRND IAMAITKFDQ FDFLIDIVPR DELKPPKRQE EVRQSVTPAE PVQYYFTLAQ QPTAVQVQGQ QQGQQTTSST TTIQPGQIII AQPQQGQTTP VTMQVGEGQQ VQIVQAQPQG QAQQAQSGTG QTMQVMQQII TNTGEIQQIP VQLNAGQLQY IRLAQPVSGT QVVQGQIQTL ATNAQQGQRN ASQGKPRRCL KETLQITQTE VQQGQQQFSQ FTDGQRNSVQ QARVSELTGE AEPREVKATG NSTPCTSSLP TTHPPSHRAG ASCVCCSQPQ QSSTSPPPSD ALQWVVVEVS GTPNQLETHR ELHAPLPGMT SLSPLHPSQQ LYQIQQVTMP AGQDLAQPMF IQSANQPSDG QAPQVTGD

  • Molecular Weight

    50.3 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

NFYC (Nuclear Transcription Factor Y Subunit C) is a key protein involved in the regulation of various gene expression processes crucial for plant growth and development, as well as responses to environmental stimuli. The NFYC gene family is highly conserved across different species, highlighting its fundamental role in cellular functions. Recent studies have revealed NFYC's involvement in numerous physiological and developmental processes, including flowering time regulation, stress response, and hormone signaling pathways. The protein functions by forming heterotrimeric complexes with NFYA and NFYB subunits, binding to CCAAT boxes in the promoter regions of various target genes, thus influencing transcriptional activity. Given its pivotal role in plant biology, research on NFYC has garnered interest not only to understand the mechanisms of transcription regulation but also to explore its potential applications in crop improvement and biotechnology. Advances in molecular techniques, such as CRISPR/Cas9 gene editing and transcriptomics, have opened new avenues for investigating NFYC's functional roles. As climate change intensifies the challenges faced by agriculture, understanding NFYC's mechanisms could facilitate the development of stress-resistant crops, ultimately contributing to food security and sustainable agricultural practices. Therefore, studying NFYC and its associated pathways offers significant potential for enhancing our understanding of plant biology and addressing global agricultural challenges.

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