Analytical Data
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Gene name
Il1f5
- Application
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Alternative Names
AI413231; Family of interleukin 1 delta; FIL1; FIL1 delta; FIL1(DELTA); FIL1D; Fil1delta; I36RA_HUMAN; IL 1 delta; IL 1 related protein 3; IL 1F5 (IL 1HY1. FIL1 delta. IL 1RP3. IL 1L1. IL 1 delta); Il 1h3; IL 1HY1; IL 1L1; IL 1ra homolog; IL 1ra homolog. IL1F5 (Canonical product IL 1F5a); IL 1RP3; IL-1 delta; IL-1-related protein 3; IL-1F5; IL-1HY1; IL-1L1; IL-1ra homolog 1; IL-1RP3; IL1F5; IL1HY1; IL1L1; IL1RP3; IL36RA; IL36RN; Interleukin 1 delta; Interleukin 1 family member 5 (delta); Interleukin 1 family member 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UBH0
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Expression Region
2-155aa
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AA Sequence
VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAGKVIKGEE ISVVPNRWLD ASLSPVILGV QGGSQCLSCG VGQEPTLTLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTVPEADQPV RLTQLPENGG WNAPITDFYF QQCD
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Molecular Weight
16.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IL1F5, also known as Interleukin 1 Family Member 5 or IL-33, is a cytokine that plays a pivotal role in the immune response and inflammation. Studies have shown that IL1F5 is involved in the regulation of Th2 responses, making it crucial in allergic diseases, asthma, and other inflammatory conditions. Researchers are increasingly focused on this cytokine due to its potential as a therapeutic target for a variety of disorders, including autoimmune diseases and cancers. The recombinant expression of IL1F5 allows for detailed investigations into its biological functions, signaling pathways, and interactions with other immune mediators. By producing and characterizing this recombinant protein, scientists aim to elucidate its role in modulating immune responses, which could lead to the development of novel therapeutic strategies. Understanding the structure-function relationship of IL1F5 through recombinant technology not only enhances our knowledge of its physiological roles but also paves the way for future clinical applications. As a result, IL1F5 is emerging as a significant focus in immunological research, potentially influencing the treatment paradigms for endocrine dysregulation and inflammatory diseases.
