Analytical Data
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Gene name
HRAS
- Application
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Alternative Names
PLAAT1;HRASLS;Phospholipase A and acyltransferase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P01112
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Expression Region
2-186aa
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AA Sequence
TEYKLVVVGA VGVGKSALTI QLIQNHFVDE YDPTIEDSYR KQVVIDGETC LLDILDTAGQ EEYSAMRDQY MRTGEGFLCV FAINNTKSFE DIHQYREQIK RVKDSDDVPM VLVGNKCDLA ARTVESRQAQ DLARSYGIPY IETSAKTRQG VEDAFYTLVR EIRQHKLRKL NPPDESGPGC MSCKC
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Molecular Weight
23 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The HRAS gene, a member of the RAS gene family, encodes a small GTPase that plays a crucial role in cell signaling pathways regulating growth and differentiation. Mutations in HRAS are frequently associated with various cancers, particularly those of the bladder, skin, and thyroid, making it a significant target for cancer research. The study of HRAS recombinant proteins is essential for understanding the molecular mechanisms by which HRAS mutations contribute to oncogenesis. Recombinant HRAS proteins, produced using techniques such as recombinant DNA technology, allow researchers to analyze the functional consequences of specific mutations, explore their interactions with downstream effector proteins, and investigate their roles in cellular signaling pathways. Additionally, these proteins serve as valuable tools in drug development, aiding in the identification of inhibitors that could potentially target HRAS-driven malignancies. By elucidating the biochemical and biophysical properties of HRAS, scientists aim to develop more effective therapeutic strategies for RAS-related cancers, which have historically proven challenging to treat due to their complex biology. The ongoing research into HRAS recombinant proteins not only enhances our fundamental understanding of this critical regulatory protein but also paves the way for innovative approaches in personalized medicine and targeted cancer therapies.
