Analytical Data
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基因名
HPSE
- Application
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别名
HPSE;HEP;HPA;HPA1;Heparanase
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y251
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表达区间
36-543aa
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氨基酸序列
QDVVDLDFFTQEPLHLVSPSFLSVTIDANLATDPRFLILLGSPKLRTLAR GLSPAYLRFGGTKTDFLIFDPKKESTFEERSYWQSQVNQDICKYGSIPPD VEEKLRLEWPYQEQLLLREHYQKKFKNSTYSRSSVDVLYTFANCSGLDLI FGLNALLRTADLQWNSSNAQLLLDYCSSKGYNISWELGNEPNSFLKKADI FINGSQLGEDFIQLHKLLRKSTFKNAKLYGPDVGQPRRKTAKMLKSFLKA GGEVIDSVTWHHYYLNGRTATKEDFLNPDVLDIFISSVQKVFQVVESTRP GKKVWLGETSSAYGGGAPLLSDTFAAGFMWLDKLGLSARMGIEVVMRQVF FGAGNYHLVDENFDPLPDYWLSLLFKKLVGTKVLMASVQGSKRRKLRVYL HCTNTDNPRYKEGDLTLYAINLHNVTKYLRLPYPFSNKQVDKYLLRPLGP HGLLSKSVQLNGLTLKMVDDQTLPPLMEKPLRPGSSLGLPAFSYSFFVIR NAKVAACI
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分子量
78 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
HPSE (Heparanase) is an endo-β-glucuronidase enzyme that plays a significant role in the degradation of heparan sulfate, a key component of the extracellular matrix. The research on HPSE has gained momentum due to its pivotal involvement in various physiological and pathological processes, including tumor metastasis, inflammation, and angiogenesis. Elevated HPSE activity has been linked to several malignancies, making it a potential target for therapeutic intervention. Recent studies have focused on elucidating the structural and functional characteristics of HPSE, particularly in its recombinant form, to understand its enzymatic mechanisms and interactions with heparan sulfate. The recombinant protein serves as a valuable tool for investigating HPSE's role in disease progression and for developing HPSE inhibitors that could inhibit its enzymatic activity, thereby opening new avenues for cancer treatment and other related disorders. The exploration of HPSE not only enhances our understanding of tumor biology but also paves the way for innovative therapeutic strategies aimed at modulating its activity for clinical benefit.
