Analytical Data
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Gene name
IFT52
- Application
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Alternative Names
IFT52; C20orf9; NGD5; CGI-53; Intraflagellar transport protein 52 homolog; Protein NGD5 homolog
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y366
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Expression Region
1-437aa
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AA Sequence
MEKELRSTILFNAYKKEIFTTNNGYKSMQKKLRSNWKIQSLKDEITSEKLNGVKLWITAGPREKFTAAEFEILKKYLDTGGDVFVMLGEGGESRFDTNINFLLEEYGIMVNNDAVVRNVYHKYFHPKEALVSSGVLNREISRAAGKAVPGIIDEESSGNNAQALTFVYPFGATLSVMKPAVAVLSTGSVCFPLNRPILAFYHSKNQGGKLAVLGSCHMFSDQYLDKEENSKIMDVVFQWLTTGDIHLNQIDAEDPEISDYMMLPYTATLSKRNRECLQESDEIPRDFTTLFDLSIFQLDTTSFHSVIEAHEQLNVKHEPLQLIQPQFETPLPTLQPAVFPPSFRELPPPPLELFDLDETFSSEKARLAQITNKCTEEDLEFYVRKCGDILGVTSKLPKDQQDAKHILEHVFFQVVEFKKLNQEHDIDTSETAFQNNF
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Molecular Weight
76.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
IFT52, or Intraflagellar Transport 52, is a central component of the intraflagellar transport (IFT) machinery, essential for the biogenesis and maintenance of cilia and flagella in eukaryotic cells. Cilia and flagella play crucial roles in various cellular processes, including motility, signaling, and sensory functions. Dysfunction in IFT components like IFT52 has been linked to a range of ciliopathies—genetic disorders caused by impaired cilia function—resulting in severe developmental and health issues. This has prompted extensive research into the structure, function, and mechanisms of IFT proteins. Recent studies on IFT52 indicate that it not only plays a role in the assembly and transport of ciliary components but also interacts with various other cellular pathways, providing insights into its multifaceted biological roles. Understanding IFT52 and its interactions is vital for elucidating the mechanisms underlying ciliopathies and could potentially lead to novel therapeutic strategies for these conditions. The research on IFT52 is thus positioned at the intersection of cell biology, genetics, and medical science, highlighting its significance in both basic research and clinical applications.
