Analytical Data
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基因名
ID4
- Application
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别名
bHLHb27; Class B basic helix-loop-helix protein 27; DNA binding protein inhibitor ID 4; DNA binding protein inhibitor ID4; DNA-binding protein inhibitor ID-4; ID 4; Id4; ID4_HUMAN; IDB4
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P47928
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表达区间
1-161aa
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氨基酸序列
MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC KAAEAAADEP ALCLQCDMND CYSRLRRLVP TIPPNKKVSK VEILQHVIDY ILDLQLALET HPALLRQPPP PAPPHHPAGT CPAAPPRTPL TALNTDPAGA VNKQGDSILC R
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分子量
16.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ID4 is a member of the inhibitor of differentiation (ID) family of proteins, which play crucial roles in regulating cell growth, differentiation, and proliferation. Initially identified for its involvement in the maintenance of multipotent progenitor cells, ID4 has garnered significant attention due to its implications in various biological processes, including embryonic development and tumorigenesis. Research has shown that ID4 functions as a dominant-negative regulator of basic helix-loop-helix (bHLH) transcription factors, thereby influencing cellular fate decisions. Its expression patterns are particularly prominent in certain cancers, suggesting a potential role in oncogenesis and serving as a biomarker for cancer diagnosis and progression. Moreover, studies have indicated that ID4 may contribute to stem cell-like properties in cancer cells, making it a target of interest for therapeutic interventions. Understanding the molecular mechanisms underlying ID4 activity could pave the way for novel approaches in regenerative medicine and cancer therapy. Thus, ongoing research into the recombinant production of ID4 protein aims to elucidate its structure-function relationships and further explore its potential as a clinical target.
