Analytical Data
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Gene name
ID3
- Application
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Alternative Names
1R21; bHLHb25; Class B basic helix-loop-helix protein 25; DNA binding protein inhibitor ID 3; DNA-binding protein inhibitor ID-3; HEIR 1; HEIR1; Helix loop helix protein HEIR 1; Helix loop helix protein HEIR1 ; Helix-loop-helix protein HEIR-1; HLH642; ID 3; ID like protein inhibitor HLH 1R21 ; ID like protein inhibitor HLH 462 ; ID-like protein inhibitor HLH 1R21; ID3; ID3_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q02535
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Expression Region
1-119aa
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AA Sequence
MKALSPVRGCYEAVCCLSERSLAIARGRGKGPAAEEPLSLLDDMNHCYSRLRELVPGVPRGTQLSQVEILQRVIDYILDLQVVLAEPAPGPPDGPHLPIQTAELAPELVISNDKRSFCH
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Molecular Weight
38.72 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ID3, a member of the inhibitor of DNA binding (ID) protein family, plays a critical role in various biological processes, including cell differentiation, proliferation, and response to stress. Its ability to antagonize basic helix-loop-helix (bHLH) transcription factors allows it to regulate gene expression dynamically during development and cellular responses. Research has revealed that ID3 is involved in key pathways such as hematopoiesis and T cell development, making it a focal point in immunological studies. Furthermore, alterations in ID3 expression have been associated with various cancers, emphasizing its importance as a potential therapeutic target. Innovative strategies are being developed to study and manipulate ID3, including the generation of recombinant ID3 proteins to better understand its structure-function relationships. Advances in recombinant DNA technology have enabled the production of purified ID3 proteins, facilitating detailed biochemical and biophysical analyses. Such studies aim to elucidate the mechanisms through which ID3 mediates its effects on cell fate decisions and contributes to tumorigenesis. Moreover, understanding the reassembly and functional dynamics of ID3 may provide insights into its interactions with other proteins and regulatory networks, paving the way for novel biomedical applications. As research continues to unravel the complexities of ID3's role in cellular processes, it holds promise for the development of targeted therapies that could leverage its unique functions in cancer and other diseases. Overall, the ongoing investigation into ID3 and its recombinant forms represents a significant frontier in molecular biology and therapeutic innovation.
