Analytical Data
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Gene name
HK4
- Application
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Alternative Names
GCK;Hexokinase-4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14397
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Expression Region
1-625aa
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AA Sequence
MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIVRLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASREGTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAVFLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIGPEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFERAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECIHTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSLTEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLKKLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKILQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSDDIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEAVRSALAGPGQKRTADPLEILEPDVQ
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Molecular Weight
70.7kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The HK4 protein, a member of the histidine kinase family, has drawn significant attention in recent years due to its vital role in various cellular processes and signal transduction pathways. Histidine kinases are pivotal in microbial and eukaryotic systems for facilitating two-component signaling mechanisms, which are crucial for responding to environmental changes. Specifically, HK4 has been implicated in regulating stress responses, cell differentiation, and pathogenicity in certain organisms. The interest in HK4 stems not only from its fundamental biological functions but also from its potential as a therapeutic target in infectious diseases and cancer. Studies have suggested that the dysregulation of HK4 can lead to various pathophysiological conditions, thus highlighting the need for a comprehensive understanding of its structure and mechanism of action. Research on HK4 encompasses various approaches, including structural biology, molecular biology, and biochemistry, aiming to elucidate its catalytic activity and interaction networks. Furthermore, the exploration of HK4's regulatory mechanisms may uncover novel strategies for modulating its activity, paving the way for the development of innovative therapeutics. Overall, the investigation of HK4 is a promising frontier in the fields of molecular biology and medicinal chemistry, with implications for enhancing our understanding of complex biological systems and devising new treatment modalities.
