Analytical Data
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Gene name
HSD3B7
- Application
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Alternative Names
HSD3B7; 3 beta-hydroxysteroid dehydrogenase type 7; 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5-C27 steroid oxidoreductase; C(27 3-beta-HSD; Cholest-5-ene-3-beta.7-alpha-diol 3-beta-dehydrogenase
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H2F3
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Expression Region
1-369aa
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AA Sequence
MADSAQAQKLVYLVTGGCGFLGEHVVRMLLQREPRLGELRVFDQHLGPWLEELKTGPVRVTAIQGDVTQAHEVAAAVAGAHVVIHTAGLVDVFGRASPKTIHEVNVQGTRNVIEACVQTGTRFLVYTSSMEVVGPNTKGHPFYRGNEDTPYEAVHRHPYPCSKALAEWLVLEANGRKVRGGLPLVTCALRPTGIYGEGHQIMRDFYRQGLRLGGWLFRAIPASVEHGRVYVGNVAWMHVLAARELEQRATLMGGQVYFCYDGSPYRSYEDFNMEFLGPCGLRLVGARPLLPYWLLVFLAALNALLQWLLRPLVLYAPLLNPYTLAVANTTFTVSTDKAQRHFGYEPLFSWEDSRTRTILWVQAATGSAQ
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Molecular Weight
67.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HSD3B7, or hydroxysteroid dehydrogenase 3 beta-like 7, is a member of the short-chain dehydrogenase/reductase superfamily and plays a critical role in steroidogenesis, particularly in the catalysis of the conversion of bile acids and steroid hormones. The enzyme's function is essential for maintaining homeostasis in lipid metabolism, and its dysregulation has been implicated in various metabolic disorders, including obesity, type 2 diabetes, and hormonal imbalances. Recent studies have highlighted the importance of HSD3B7 in liver function and its potential as a therapeutic target for treating liver diseases and metabolic syndromes. The recombinant HSD3B7 protein is produced to facilitate structural and functional studies, which can deepen our understanding of its enzymatic mechanisms and substrate specificity. Advancements in recombinant protein technologies have made it feasible to produce HSD3B7 in sufficient quantities for in vitro analysis, allowing researchers to explore its potential roles in drug development and metabolic regulation. Consequently, understanding the biological and biochemical characteristics of HSD3B7 through recombinant protein research can pave the way for novel therapeutic strategies aimed at mitigating the impacts of metabolic diseases and enhancing our understanding of steroid hormone biosynthesis.
