Analytical Data
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基因名
GUSb
- Application
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别名
GUSb；Beta-glucuronidase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P08236
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表达区间
23-651aa
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氨基酸序列
LQGGMLYPQESPSRECKELDGLWSFRADFSDNRRRGFEEQWYRRPLWESG PTVDMPVPSSFNDISQDWRLRHFVGWVWYEREVILPERWTQDLRTRVVLR IGSAHSYAIVWVNGVDTLEHEGGYLPFEADISNLVQVGPLPSRLRITIAI NNTLTPTTLPPGTIQYLTDTSKYPKGYFVQNTYFDFFNYAGLQRSVLLYT TPTTYIDDITVTTSVEQDSGLVNYQISVKGSNLFKLEVRLLDAENKVVAN GTGTQGQLKVPGVSLWWPYLMHERPAYLYSLEVQLTAQTSLGPVSDFYTL PVGIRTVAVTKSQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNL LRWLGANAFRTSHYPYAEEVMQMCDRYGIVVIDECPGVGLALPQFFNNVS LHHHMQVMEEVVRRDKNHPAVVMWSVANEPASHLESAGYYLKMVIAHTKS LDPSRPVTFVSNSNYAADKGAPYVDVICLNSYYSWYHDYGHLELIQLQLA TQFENWYKKYQKPIIQSEYGAETIAGFHQDPPLMFTEEYQKSLLEQYHLG LDQKRRKYVVGELIWNFADFMTEQSPTRVLGNKKGIFTRQRQPKSAAFLL RERYWKIANETRYPHSVAKSQCLENSLFTHHHHHH
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分子量
73 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GUSb, which stands for β-glucuronidase B, is a variant of the glycosyl hydrolase enzyme that has garnered significant attention in both basic and applied research fields. Originally derived from various sources, such as bacteria and plants, GUSb is known for its ability to catalyze the hydrolysis of glucuronides, thereby playing a crucial role in metabolic processes and biotransformation. Its significance extends to environmental microbiology, where it is utilized as a biomarker for microbial activity and as an indicator for soil health. In the context of genetic engineering and synthetic biology, GUSb serves as a reporter gene, facilitating the monitoring of gene expression and the effectiveness of transformation processes in plant and animal models. Recent studies have focused on optimizing GUSb for enhanced stability and activity, which hold potential implications in biopharmaceuticals and biotechnology applications. The exploration of GUSb's structure-function relationships through molecular biology techniques has provided insights into its catalytic mechanisms, paving the way for innovations in enzyme engineering and therapeutic interventions. Overall, GUSb represents a valuable tool in both academic research and industrial applications, driving advancements in biotechnology, agriculture, and environmental science.
