Analytical Data
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Gene name
HRMT1L3
- Application
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Alternative Names
2010005E20Rik; 2410018A17Rik; AL033309; ANM3_HUMAN; Heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 3; Heterogeneous nuclear ribonucleoprotein methyltransferase like 3; Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 3; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3; HMT1 hnRNP methyltransferase like 3; HMT1 hnRNP methyltransferase like 3 S. cerevisiae; HMT1-like 3; hnRNP methyltransferase like 3; HRMT1L3
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60678
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Expression Region
2-531aa
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AA Sequence
CSLASGATG GRGAVENEED LPELSDSGDE AAWEDEDDAD LPHGKQQTPC LFCNRLFTSA EETFSHCKSE HQFNIDSMVH KHGLEFYGYI KLINFIRLKN PTVEYMNSIY NPVPWEKEEY LKPVLEDDLL LQFDVEDLYE PVSVPFSYPN GLSENTSVVE KLKHMEARAL SAEAALARAR EDLQKMKQFA QDFVMHTDVR TCSSSTSVIA DLQEDEDGVY FSSYGHYGIH EEMLKDKIRT ESYRDFIYQN PHIFKDKVVL DVGCGTGILS MFAAKAGAKK VLGVDQSEIL YQAMDIIRLN KLEDTITLIK GKIEEVHLPV EKVDVIISEW MGYFLLFESM LDSVLYAKNK YLAKGGSVYP DICTISLVAV SDVNKHADRI AFWDDVYGFK MSCMKKAVIP EAVVEVLDPK TLISEPCGIK HIDCHTTSIS DLEFSSDFTL KITRTSMCTA IAGYFDIYFE KNCHNRVVFS TGPQSTKTHW KQTVFLLEKP FSVKAGEALK GKVTVHKSKK DPRSLTVTLT LNNSTQTYGL Q
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Molecular Weight
59.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HRMT1L3, a member of the lysine methyltransferase family, is primarily implicated in the methylation of histone proteins, a crucial process in the regulation of gene expression and chromatin structure. The enzyme is known for its role in epigenetic modifications, which influence various biological processes, including cellular differentiation, proliferation, and response to environmental stimuli. Dysregulation of HRMT1L3 has been linked to various diseases, including cancers, where altered methylation patterns can lead to abnormal gene expression. Given its significance in epigenetics and potential as a therapeutic target, the study of HRMT1L3 recombinant protein is essential for understanding its functional mechanisms and interactions within the cell. Research into the structure and activity of HRMT1L3 not only sheds light on the molecular basis of its enzymatic function but also paves the way for the development of novel inhibitors that could modulate its activity. As such, the recombinant production of HRMT1L3 allows for detailed biochemical studies and the exploration of its role in chromatin dynamics and gene regulation, offering insights that could contribute to targeted cancer therapies and advancements in epigenetic research.
