Analytical Data
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Gene name
GSR
- Application
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Alternative Names
GSR;GLUR;GRD1;Glutathione reductase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P00390
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Expression Region
106-522aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSM PKKVMWNTAV HSEFMHDHAD YGFPSCEGKF NWRVIKEKRD AYVSRLNAIY QNNLTKSHIE IIRGHAAFTS DPKPTIEVSG KKYTAPHILI ATGGMPSTPH ESQIPGASLG ITSDGFFQLE ELPGRSVIVG AGYIAVEMAG ILSALGSKTS LMIRHDKVLR SFDSMISTNC TEELENAGVE AMACRQ EPQPQGPPPA AGAVASYDYL VIGGGSGGLA SARRAAELGA RAAVVESHKL GGTCVNVGCV VLKFSQVKEV KKTLSGLEVS MVTAVPGRLP VMTMIPDVDC LLWAIGRVPN TKDLSLNKLG IQTDDKGHII VDEFQNTNVK GIYAVGDVCG KALLTPVAIA AGRKLAHRLF EYKEDSKLDY NNIPTVVFSH PPIGTVGLTE DEAIHKYGIE NVKTYSTSFT PMYHAVTKRK TKCVMKMVCA NKEEKVVGIH MQGLGCDEML QGFAVAVKMG ATKADFDNTV AIHPTSSEEL VTLR
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Molecular Weight
54 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GSR, or Glutathione Reductase, is a significant enzyme that plays a crucial role in cellular antioxidant defense by catalyzing the reduction of glutathione disulfide to its sulfhydryl form, glutathione. Understanding the structure and function of GSR is vital for elucidating its roles in redox homeostasis, cellular proliferation, and apoptosis, as well as its implications in various diseases, including cancer and neurodegenerative disorders. Research into GSR recombinant proteins has gained momentum due to their potential applications in biotechnology and medicine, allowing for the investigation of enzyme kinetics, substrate specificity, and interaction with other biomolecules. Furthermore, GSR is a target for developing inhibitors that could serve as therapeutic agents, particularly in conditions involving oxidative stress. The expression of GSR as a recombinant protein in suitable host systems enables detailed studies of its biochemical properties and interactions, paving the way for novel therapeutic developments. Overall, the study of recombinant GSR not only enhances our understanding of this essential enzyme's biological functions but also provides a pathway for innovative strategies in combating oxidative stress-related diseases.











