Analytical Data
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基因名
PMT3
- Application
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别名
PMT3；Dolichyl-phosphate-mannose--Protein mannosyltransferase 3
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P47190
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表达区间
304-602aa
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氨基酸序列
LLSNSGPGDSTMPSLFQASLNGTKVGKGPRDVALGSSIISIKNQALGGALLHSHVQPFPEGSEQQQVTVYGYSDANNEWFFQRIRGVEPWTDAENKTIEFVKGGEMYRLMHRLTGKNLHTHEVPAPISKSEYEVSAYGDVDLGDYKDNWIIEIVEQVGEEDPTLLHPLSTSFRIKNSILGCYLAQSGKHLPEWGFRQGEVVCLKHASKRDKRTWWNIETHENERLPQGEDFVYPKTSFFRNFMQLNSAMMATNNALVPNPEKFDGIASSAWQWPTLNVGVRLCEWSEKSIKYFLLGSPA
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分子量
39.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PMT3 is a phosphomannomutase involved in the biosynthesis of glycoproteins and glycosylation processes in various organisms, particularly in yeast and higher plants. Research on PMT3 has gained attention due to its essential role in the conversion of mannose-6-phosphate to mannose-1-phosphate, a crucial step in the synthesis of GDP-mannose, which is a fundamental building block for the synthesis of polysaccharides and glycoconjugates. Dysregulation of glycosylation pathways can lead to diverse diseases, including certain cancers and congenital disorders, making the study of PMT3 not only relevant for basic biology but also for therapeutic applications. Understanding the enzymatic mechanisms by which PMT3 operates and its regulatory pathways can reveal potential targets for drug development aimed at correcting glycosylation defects. Furthermore, research into PMT3's structure and function can enhance our knowledge of how glycosylation affects cell signaling, immune responses, and protein stability, contributing to the development of more effective biopharmaceuticals. Given the increasing significance of glycosylation in various biological processes and its implications in human health, PMT3 stands out as a key protein of interest in the field of glycobiology and biochemistry.
