Analytical Data
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Gene name
PMT3
- Application
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Alternative Names
PMT3；Dolichyl-phosphate-mannose--Protein mannosyltransferase 3
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P47190
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Expression Region
304-602aa
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AA Sequence
LLSNSGPGDSTMPSLFQASLNGTKVGKGPRDVALGSSIISIKNQALGGALLHSHVQPFPEGSEQQQVTVYGYSDANNEWFFQRIRGVEPWTDAENKTIEFVKGGEMYRLMHRLTGKNLHTHEVPAPISKSEYEVSAYGDVDLGDYKDNWIIEIVEQVGEEDPTLLHPLSTSFRIKNSILGCYLAQSGKHLPEWGFRQGEVVCLKHASKRDKRTWWNIETHENERLPQGEDFVYPKTSFFRNFMQLNSAMMATNNALVPNPEKFDGIASSAWQWPTLNVGVRLCEWSEKSIKYFLLGSPA
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Molecular Weight
39.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PMT3 is a phosphomannomutase involved in the biosynthesis of glycoproteins and glycosylation processes in various organisms, particularly in yeast and higher plants. Research on PMT3 has gained attention due to its essential role in the conversion of mannose-6-phosphate to mannose-1-phosphate, a crucial step in the synthesis of GDP-mannose, which is a fundamental building block for the synthesis of polysaccharides and glycoconjugates. Dysregulation of glycosylation pathways can lead to diverse diseases, including certain cancers and congenital disorders, making the study of PMT3 not only relevant for basic biology but also for therapeutic applications. Understanding the enzymatic mechanisms by which PMT3 operates and its regulatory pathways can reveal potential targets for drug development aimed at correcting glycosylation defects. Furthermore, research into PMT3's structure and function can enhance our knowledge of how glycosylation affects cell signaling, immune responses, and protein stability, contributing to the development of more effective biopharmaceuticals. Given the increasing significance of glycosylation in various biological processes and its implications in human health, PMT3 stands out as a key protein of interest in the field of glycobiology and biochemistry.
