Analytical Data
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Gene name
INSIG2
- Application
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Alternative Names
INSIG2；Insulin-induced gene 2 Protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y5U4
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Expression Region
1-225aa
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AA Sequence
MAEGETESPG PKKCGPYISS VTSQSVNLMI RGVVLFFIGV FLALVLNLLQ IQRNVTLFPP DVIASIFSSA WWVPPCCGTA SAVIGLLYPC IDRHLGEPHK FKREWSSVMR CVAVFVGINH ASAKVDFDNN IQLSLTLAAL SIGLWWTFDR SRSGFGLGVG IAFLATVVTQ LLVYNGVYQY TSPDFLYVRS WLPCIFFAGG ITMGNIGRQL AMYECKVIAE KSHQE
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Molecular Weight
32 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
INSIG2 (Insulin Induced Gene 2) is a crucial protein involved in lipid metabolism and cholesterol homeostasis, functioning primarily in the regulation of sterol regulatory element-binding proteins (SREBPs). It serves as a key player in maintaining cellular lipid levels by facilitating the retention of SREBPs in the endoplasmic reticulum, thereby preventing their maturation and activation of lipogenic genes. The study of INSIG2, particularly through the production of recombinant INSIG2 protein, has garnered significant interest due to its potential implications in metabolic disorders, including obesity and type 2 diabetes. Recent research has suggested that genetic variations in the INSIG2 gene may also be associated with obesity susceptibility in humans, underscoring the importance of this protein in understanding metabolic pathways. The recombinant protein can be utilized in various biochemical assays to explore its molecular interactions and functional mechanisms, contributing to the development of therapeutic strategies aimed at targeting dysregulated lipid metabolism. Given its role in energy homeostasis, further investigation of INSIG2 and its interactions could provide insights into novel approaches for treating metabolic diseases and highlight its potential as a biomarker for obesity-related conditions.
