Analytical Data
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基因名
glpK
- Application
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别名
glpK;Glycerol kinase
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A6F3
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表达区间
2-502aa
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氨基酸序列
TEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE
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分子量
60.1kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Glucose-6-phosphate kinase (glpK) is an essential enzyme involved in the regulation of glucose metabolism, particularly in the glycolytic pathway. It catalyzes the phosphorylation of glucose-6-phosphate, playing a critical role in energy production and metabolic control in various organisms, including bacteria, plants, and animals. Research on glpK has gained significance due to its potential applications in biotechnology and medicine, such as its role in metabolic engineering, the development of antimicrobial agents, and understanding metabolic disorders. The recombinant production of glpK allows for the detailed study of its structure, function, and regulation, enabling researchers to explore how alterations in this enzyme can affect metabolic pathways. Furthermore, the availability of large quantities of pure glpK protein facilitates crystallographic studies, which can provide insights into its active site and interaction with substrates or inhibitors. As metabolic diseases and antibiotic resistance continue to pose significant public health challenges, understanding glpK function and its regulatory mechanisms could lead to novel therapeutic strategies and expanded applications in metabolic engineering for biofuel production and bioremediation. Thus, the research on glpK recombinant protein not only enhances our fundamental knowledge of metabolic processes but also holds promise for practical applications across multiple fields.
