Analytical Data
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Gene name
HEY1
- Application
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Alternative Names
Basic helix loop helix protein OAF1; bHLHb31; Cardiovascular helix loop-helix factor 2; Cardiovascular helix-loop-helix factor 2; CHF-2; CHF2; Class B basic helix-loop-helix protein 31; Hairy and enhancer of split related protein 1; Hairy and enhancer of split-related protein 1; Hairy enhancer-of-split related with YRPW motif protein 1; Hairy related transcription factor 1; Hairy-related transcription factor 1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y5J3
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Expression Region
1-304aa
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AA Sequence
MKRAHPEYSSSDSELDETIEVEKESADENGNLSSALGSMSPTTSSQILARKRRRGIIEKRRRDRINNSLSELRRLVPSAFEKQGSAKLEKAEILQMTVDHLKMLHTAGGKGYFDAHALAMDYRSLGFRECLAEVARYLSIIEGLDASDPLRVRLVSHLNNYASQREAASGAHAGLGHIPWGTVFGHHPHIAHPLLLPQNGHGNAGTTASPTEPHHQGRLGSAHPEAPALRAPPSGSLGPVLPVVTSASKLSPPLLSSVASLSAFPFSFGSFHLLSPNALSPSAPTQAANLGKPYRPWGTEIGAF
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Molecular Weight
59.07 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HEY1 (Heart and neural crest derivatives expressed 1) is a member of the basic helix-loop-helix (bHLH) transcription factor family, playing a crucial role in various developmental processes, including neurogenesis, cardiogenesis, and the maintenance of stem cell pluripotency. Research on HEY1 has gained momentum due to its involvement in key signaling pathways, particularly Notch signaling, which is essential for cell fate determination and tissue homeostasis. Aberrant regulation of HEY1 has been linked to several pathological conditions, including cancer, where it can act as an oncogene or tumor suppressor depending on the cellular context. The study of HEY1 recombinant proteins allows researchers to investigate its functional properties, including DNA binding, protein interactions, and influence on transcriptional activity. By producing HEY1 in a recombinant form, scientists can facilitate assays to elucidate its role in cellular processes and identify potential therapeutic targets. The characterization of HEY1 recombinant proteins also holds promise for developing novel strategies in regenerative medicine and gene therapy, emphasizing the importance of understanding this transcription factor's biology in both health and disease.
