Analytical Data
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Gene name
GDF5
- Application
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Alternative Names
GDF5；BMP14；CDMP1;Growth/differentiation factor 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P43026
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Expression Region
382-501aa
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AA Sequence
MAPLATRQGK RPSKNLKARC SRKALHVNFK DMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ TLMNSMDPES TPPTCCVPTR LSPISILFIDSA NNVVYKQY EDMVVESCGC R
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Molecular Weight
55 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GDF5 (Growth Differentiation Factor 5) is a member of the transforming growth factor-beta (TGF-β) superfamily, playing a crucial role in bone and cartilage development, as well as in the regeneration of these tissues. Research into GDF5 recombinant protein has intensified due to its potential applications in regenerative medicine, particularly for musculoskeletal disorders such as osteoarthritis and cartilage injuries. GDF5 promotes chondrogenesis and bone repair by enhancing the proliferation and differentiation of mesenchymal stem cells into chondrocytes and osteoblasts. Studies have shown that GDF5 can stimulate the expression of key extracellular matrix components, which are vital for maintaining cartilage integrity and function. Furthermore, the therapeutic use of GDF5 in tissue engineering has been explored, owing to its ability to induce the formation of new cartilage and bone in animal models. However, challenges remain in optimizing delivery methods and ensuring sustained release at the injury site. The production of GDF5 as a recombinant protein allows for the generation of high-purity and biologically active forms, facilitating a deeper understanding of its biological functions and paving the way for clinical applications. Ongoing research focuses on elucidating the molecular mechanisms underpinning GDF5's actions and exploring combined therapies to enhance its efficacy in tissue repair and regeneration. Overall, the investigation into GDF5 recombinant protein holds promise for advancing therapeutic strategies in orthopedic and regenerative medicine.
