Analytical Data
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Gene name
Gna12
- Application
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Alternative Names
Gna12；Guanine nucleotide-binding Protein subunit alpha-12
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P27600
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Expression Region
1-379aa
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AA Sequence
MSGVVRTLSRCLLPAEAGARERRAGAARDAEREARRRSRDIDALLARERRAVRRLVKILLLGAGESGKSTFLKQMRIIHGREFDQKALLEFRDTIFDNILKGSRVLVDARDKLGIPWQHSENEKHGMFLMAFENKAGLPVEPATFQLYVPALSALWRDSGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYFPSKQDILLARKATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFDGITSILFMVSSSEYDQVLMEDRRTNRLVESMNIFETIVNNKLFFNVSIILFLNKMDLLVEKVKSVSIKKHFPDFKGDPHRLEDVQRYLVQCFDRKRRNRSKPLFHHFTTAIDTENIRFVFHAVKDTILQENLKDIMLQ
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Molecular Weight
51.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Gna12 is a member of the G-protein family, specifically categorized as a heterotrimeric GTP-binding protein, which plays a critical role in various cellular signaling pathways. The research background of Gna12 centers on its involvement in regulating several physiological processes, including cell proliferation, differentiation, and migration. Dysregulation of Gna12 has been linked to a variety of diseases, including cancer and cardiovascular disorders, thereby highlighting its potential as a therapeutic target. Several studies have indicated that Gna12 mediates its effects through interactions with downstream effectors such as Rho family GTPases, influencing cytoskeletal dynamics and cell adhesion. Additionally, Gna12’s role in signal transduction pathways such as G protein-coupled receptor (GPCR) signaling further underscores its importance in cellular communication. Recent advancements in recombinant protein technology have facilitated the production of purifed Gna12 proteins, enabling in-depth biochemical and structural studies that aim to elucidate its functional mechanisms. This research not only enhances our understanding of Gna12’s contribution to normal cellular functions but also assists in uncovering its potential roles in pathological conditions, paving the way for new therapeutic strategies targeting Gna12-mediated pathways. As such, the investigation of Gna12 recombinant proteins represents a significant avenue in both basic and applied biomedical research.
