Analytical Data
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Gene name
RNH1
- Application
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Alternative Names
RNH1；RNH1；Ribonuclease H1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P13489
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Expression Region
2-461aa
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AA Sequence
SLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDDCGLTEARCKDISSALRVNPALAELNLRSNELGDVGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVLSSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDPQCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKELTVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCGVTSDNCRDLCGIVASKASLRELALGSNKLGDVGMAELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVLRAKESLKELSLAGNELGDEGARLLCETLLEPGCQLESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISNNRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDSSCSSLAATLLANHSLRELDLSNNCLGDAGILQLVESVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKPSLRVIS
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Molecular Weight
53.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNH1, or ribonuclease inhibitor 1, is a crucial protein in the regulation of ribonuclease activity within the cytoplasm, playing a significant role in RNA metabolism and cellular homeostasis. Its primary function is to inhibit ribonucleases such as RNase A, thereby preventing the degradation of RNA molecules, which is vital for various cellular processes, including protein synthesis, mRNA stability, and gene expression regulation. Research into RNH1 has gained momentum due to its implications in several diseases, including cancer and autoimmune disorders, where altered RNA metabolism is often observed. Additionally, RNH1 has emerged as a potential therapeutic target; understanding its structure and function could lead to the development of specific inhibitors or enhancers that modulate ribonuclease activity, providing insights into novel treatment strategies. Recent advances in recombinant protein technology allow for the production of RNH1 in sufficient quantities for detailed biochemical studies, enabling researchers to explore its interactions with ribonucleases and other RNA-binding proteins. This research not only furthers our understanding of RNA biology but may also pave the way for innovative approaches in the treatment of diseases associated with dysregulated RNA metabolism.
