Analytical Data
-
Gene name
GADD153
- Application
-
Alternative Names
GADD153；CHOP；CHOP10;DNA damage-inducible transcript 3 Protein
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P35638
-
Expression Region
1-169aa
-
AA Sequence
MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GADD153, also known as CHOP (C/EBP homologous protein), is a member of the C/EBP family of transcription factors that plays a crucial role in the cellular stress response, particularly in the context of the endoplasmic reticulum (ER) stress pathway. It is upregulated in response to various stressors, including hypoxia, nutrient deprivation, and oxidative stress, leading to the activation of apoptosis and cell cycle arrest. Research surrounding GADD153 has gained significant attention due to its implications in various pathological conditions, including cancer, neurodegenerative diseases, and cardiovascular disorders. As a mediator of the unfolded protein response (UPR), GADD153 acts as a pivotal regulator that determines cell fate during stress. The recombinant expression of GADD153 allows for in-depth studies of its structural properties and functional roles, thereby providing insights into its regulatory mechanisms. Investigating this protein's behavior not only enhances our understanding of the UPR but also reveals potential therapeutic targets for diseases associated with dysregulated cellular stress responses. Furthermore, GADD153's involvement in modulating inflammatory responses and apoptosis positions it as a critical factor in the development and progression of diseases, making it a significant subject of biomedical research. Therefore, the study of GADD153 recombinant protein not only contributes to basic science but also holds promise for translational applications in disease prevention and treatment strategies.
