Analytical Data
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Gene name
GAD1
- Application
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Alternative Names
GAD1；GAD；GAD67;Glutamate decarboxylase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99259-1
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Expression Region
1-594aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMASSTPS SSATSSNAGA DPNTTNLRPT TYDTWCGVAH GCTRKLGLKI CGFLQRTNSL EEKSRLVSAF KERQSSKNLL SCENSDRDAR FRRTETDFSN LFARDLLPAK NGEEQTVQFL LEVVDILLNY VRKTFDRSTK VLDFHHPHQL LEGMEGFNLE LSDHPESLEQ ILVDCRDTLK YGVRTGHPRF FNQLSTGLDI IGLAGEWLTS TANTNMFTYE IAPVFVLMEQ ITLKKMREIV GWSSKDGDGI FSPGGAISNM YSIMAARYKY FPEVKTKGMA AVPKLVLFTS EQSHYSIKKA GAALGFGTDN VILIKCNERG KIIPADFEAK ILEAKQKGYV PFYVNATAGT TVYGAFDPIQ EIADICEKYN LWLHVDAAWG GGLLMSRKHR HKLNGIERAN SVTWNPHKMM GVLLQCSAIL VKEKGILQGC NQMCAGYLFQ PDKQYDVSYD TGDKAIQCGR HVDIFKFWLM WKAKGTVGFE NQINKCLELA EYLYAKIKNR EEFEMVFNGE PEHTNVCFWY IPQSLRGVPD SPQRREKLHK VAPKIKALMM ESGTTMVGYQ PQGDKANFFR MVISNPAATQ SDIDFLIEEI ERLGQDL
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Molecular Weight
69 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GAD1, or glutamic acid decarboxylase 1, is a key enzyme responsible for the synthesis of gamma-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system. The significance of GAD1 in neuronal signaling and its involvement in various neurological conditions, such as epilepsy, anxiety disorders, and schizophrenia, has sparked considerable interest in its study. Recombinant GAD1 protein has become a valuable tool for researchers aiming to understand its biochemical properties and functional mechanisms. By expressing GAD1 in heterologous systems, scientists can investigate its enzymatic activity, regulatory mechanisms, and interactions with other proteins. The study of recombinant GAD1 not only enhances our understanding of GABAergic signaling but also holds potential for developing targeted therapies for GABA-related disorders. Additionally, examining the structure-function relationship of GAD1 through recombinant protein techniques may reveal insights into its role in neurodevelopment and neurotransmission. Overall, the research surrounding GAD1 recombinant protein serves as a crucial bridge between basic neuroscience and clinical application, aiming to address the pressing need for effective treatments for GABAergic dysfunctions.
