Analytical Data
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Gene name
GPR4
- Application
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Alternative Names
GPR4; G-protein coupled receptor 4; G-protein coupled receptor 6C.l; GPR6C.l
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P46093
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Expression Region
1-362aa
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AA Sequence
MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQRNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMEGWVAWMNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ
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Molecular Weight
67.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GPR4, a member of the G protein-coupled receptor (GPCR) family, has garnered significant attention in recent years due to its potential role in human physiology and pathology. This receptor is notably activated by changes in extracellular pH, suggesting its involvement in various physiological processes such as acid-base homeostasis and inflammation. Research has indicated that GPR4 may play a crucial role in regulating cell proliferation, migration, and survival, which are fundamental processes in cancer progression and metastasis. Additionally, GPR4's expression may vary in different tissues, potentially influencing its physiological roles and implications in diverse diseases. The investigation into GPR4 recombinant proteins enables researchers to elucidate its structure-function relationships, signaling mechanisms, and potential therapeutic targets. The generation of these recombinant proteins facilitates in vitro studies that can explore GPR4's interaction with ligands and downstream signaling pathways. Furthermore, understanding the functional dynamics of GPR4 could unveil new strategies for drug development, particularly in targeting cancers or inflammatory conditions where this receptor is implicated. As a relatively underexplored GPCR, GPR4 represents a promising avenue for innovative research aimed at leveraging its physiological roles for therapeutic benefits. Overall, the study of GPR4 recombinant proteins is crucial for advancing our understanding of this receptor's contributions to health and disease, ultimately guiding the development of novel intervention strategies.
