Analytical Data
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Gene name
DYNC1I1
- Application
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Alternative Names
DYNC1I1；DNCI1；DNCIC1；Cytoplasmic dynein 1 intermediate chain 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14576
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Expression Region
1-645aa
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AA Sequence
MSDKSDLKAELERKKQRLAQIREEKKRKEEERKKKEADMQQKKEPVQDDSDLDRKRRETEALLQSIGISPEPPLVQPLHFLTWDTCYFHYLVPTPMSPSSKSVSTPSEAGSQDSGDLGPLTRTLQWDTDPSVLQLQSDSELGRRLHKLGVSKVTQVDFLPREVVSYSKETQTPLATHQSEEDEEDEEMVESKVGQDSELENQDKKQEVKEAPPRELTEEEKQQILHSEEFLIFFDRTIRVIERALAEDSDIFFDYSGRELEEKDGDVQAGANLSFNRQFYDEHWSKHRVVTCMDWSLQYPELMVASYNNNEDAPHEPDGVALVWNMKFKKTTPEYVFHCQSSVMSVCFARFHPNLVVGGTYSGQIVLWDNRSHRRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLITVSTDGKMCSWSLDMLSTPQESMELVYNKSKPVAVTGMAFPTGDVNNFVVGSEEGTVYTACRHGSKAGIGEVFEGHQGPVTGINCHMAVGPIDFSHLFVTSSFDWTVKLWTTKHNKPLYSFEDNADYVYDVMWSPVHPALFACVDGMGRLDLWNLNNDTEVPTASVAIEGASALNRVRWAQAGKEVAVGDSEGRIWVYDVGELAVPHNDEWTRFARTLVEIRANRADSEEEGTVELSA
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Molecular Weight
75.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DYNC1I1, or cytoplasmic dynein 1 heavy chain 1, is a critical component of the dynein motor protein complex, which is essential for many cellular processes, including organelle transport, mitosis, and endocytosis. Research into DYNC1I1 has gained momentum due to its significant role in cellular machinery and its association with various neurodegenerative diseases, such as Alzheimer's and Huntington's disease, where disrupted intracellular transport leads to cellular dysfunction. Genetic mutations or dysregulation in DYNC1I1 expression can impair dynein function, resulting in compromised neuronal health and tissue homeostasis. As a result, the study of DYNC1I1 recombinant proteins offers valuable insights into the mechanistic pathways of motor protein function and its implications in disease models. By expressing and characterizing DYNC1I1 in a recombinant system, researchers can investigate the structural and functional properties of this protein, evaluate its interactions with other cellular components, and screen potential therapeutic compounds that could restore normal dynein activity. Ultimately, understanding DYNC1I1’s biology is pivotal for developing targeted interventions in diseases linked to dynein malfunction, making it a focal point of cellular and biomedical research.
