Analytical Data
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Gene name
HS6ST3
- Application
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Alternative Names
HS6ST3；Heparan-sulfate 6-O-sulfotransferase 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IZP7
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Expression Region
1-471aa
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AA Sequence
MDERFNKWLLTPVLTLLFVVIMYQYVSPSCTSSCTNFGEQPRAGEAGPPAVPGPARRAQAPPEEWERRPQLPPPPRGPPEGPRGAAAPEEEDEEPGDPREGEEEEEEDEPDPEAPENGSLPRFVPRFNFSLKDLTRFVDFNIKGRDVIVFLHIQKTGGTTFGRHLVKNIRLEQPCSCKAGQKKCTCHRPGKKETWLFSRFSTGWSCGLHADWTELTNCVPAIMEKKDCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCTYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVEINEGARQRIEDLNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRW
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Molecular Weight
54.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HS6ST3, or Heparan sulfate 6-O-sulfotransferase 3, is an essential enzyme involved in the biosynthesis of heparan sulfate, a glycosaminoglycan that plays a crucial role in various biological processes, including cell signaling, embryonic development, and wound healing. This enzyme specifically introduces sulfate groups at the 6-position of glucosamine residues within heparan sulfate chains, which is vital for modulating interactions between heparan sulfate and a wide range of proteins, including growth factors, morphogens, and extracellular matrix components. Research into HS6ST3 has gained momentum due to its implications in health and disease, particularly its potential role in cancer metastasis, viral infections, and various genetic disorders. Understanding HS6ST3's function and regulatory mechanisms can provide insights into therapeutic strategies aimed at targeting heparan sulfate-mediated pathways. Additionally, recombinant expression of HS6ST3 provides a valuable tool for studying its enzymatic activity, substrate specificity, and interactions with other biomolecules, thereby enhancing our overall comprehension of heparan sulfate chemistry and its physiological roles. Overall, the characterization of HS6ST3 as a recombinant protein opens avenues for both fundamental research and future clinical applications, emphasizing the importance of this enzyme in the intricate network of cell signaling and tissue dynamics.
