Analytical Data
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Gene name
rpsG
- Application
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Alternative Names
rpsG；spoIIIG；RNA polymerase sigma-G factor
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P02359
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Expression Region
2-179aa
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AA Sequence
PRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRWLSLRSFSHQAGASSKQPALGYLN
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Molecular Weight
23.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The rpsG gene, which encodes the ribosomal protein S7, plays a crucial role in the assembly and function of the ribosome, the cellular machinery responsible for protein synthesis. Researching rpsG is vital because ribosomal proteins are essential for maintaining the integrity and efficiency of the translational process, and any alterations in these proteins can lead to various developmental and metabolic disorders. Moreover, studies on rpsG and its encoded protein have implications for understanding antibiotic resistance, as some antibiotics target ribosomal proteins to inhibit bacterial growth. Recombinant protein technology offers a powerful approach to investigate the structure and function of the RpsG protein in detail. By producing rpsG in a heterologous system, researchers can analyze its interactions with other ribosomal components and characterize its biochemical properties. This understanding can shed light on the evolutionary adaptations of ribosomes across different organisms and may contribute to the development of novel antimicrobial agents. Additionally, studying rpsG can provide insights into the mechanisms of ribosome biogenesis and the regulation of gene expression at the translational level. Overall, the exploration of rpsG through recombinant protein studies is essential for elucidating fundamental biological processes and addressing critical challenges in microbiology and medicine.
